Orthogonal replication with optogenetic selection evolves yeast JEN1 into a mevalonate transporter
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DOI: 10.1038/s44320-025-00113-5
Abstract: The in vivo continuous evolution system OrthoRep (orthogonal replication) is a powerful strategy for rapid enzyme evolution in Saccharomyces cerevisiae that diversifies genes at a rate exceeding the endogenous genome mutagenesis rate by several orders… read more here.
Keywords: evolution; mevalonate; orthogonal replication; selection ... See more keywords
Establishing a synthetic orthogonal replication system enables accelerated evolution in E. coli
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DOI: 10.1126/science.adk1281
Abstract: The evolution of new function in living organisms is slow and fundamentally limited by their critical mutation rate. Here, we established a stable orthogonal replication system in Escherichia coli. The orthogonal replicon can carry diverse… read more here.
Keywords: evolution; replication; replication system; coli ... See more keywords