219 Relationship between host-genetics and the vaginal microbiome in commercial gilts
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Animal Science"
DOI: 10.1093/jas/skz258.086
Abstract: The objective of this study was to investigate host-genetic contributions to the vaginal microbiome of commercial gilts vaccinated for Porcine Reproductive and Respiratory Syndrome (PRRS). Vaginal swab samples (n = 576) from 308 F1 gilts… read more here.
Keywords: genetics; otus; microbiome commercial; vaginal microbiome ... See more keywords
OptiFit: an Improved Method for Fitting Amplicon Sequences to Existing OTUs
Sign Up to like & getrecommendations! Published in 2022 at "mSphere"
DOI: 10.1128/msphere.00916-21
Abstract: Advancements in DNA sequencing technology have allowed researchers to affordably generate millions of sequence reads from microorganisms in diverse environments. Efficient and robust software tools are needed to assign microbial sequences into taxonomic groups for… read more here.
Keywords: reference; amplicon sequences; data sets; optifit ... See more keywords
Bacterial Communities in the Feces of Laboratory Reared Gampsocleis gratiosa (Orthoptera: Tettigoniidae) across Different Developmental Stages and Sexes
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DOI: 10.3390/insects13040361
Abstract: Simple Summary Many insects host a diverse gut microbial community, ranging from pathogenic to obligate mutualistic organisms. Little is known about the bacteria associated with katydids. Gampsocleis gratiosa (Orthoptera, Tettigoniidae) is an economically important singing… read more here.
Keywords: otus; gampsocleis gratiosa; developmental stages; laboratory reared ... See more keywords
Random sampling causes the low reproducibility of rare eukaryotic OTUs in Illumina COI metabarcoding
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DOI: 10.7717/peerj.3006
Abstract: DNA metabarcoding, the PCR-based profiling of natural communities, is becoming the method of choice for biodiversity monitoring because it circumvents some of the limitations inherent to traditional ecological surveys. However, potential sources of bias that… read more here.
Keywords: sample; rare otus; otus; reproducibility ... See more keywords
Accuracy of microbial community diversity estimated by closed- and open-reference OTUs
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DOI: 10.7717/peerj.3889
Abstract: Next-generation sequencing of 16S ribosomal RNA is widely used to survey microbial communities. Sequences are typically assigned to Operational Taxonomic Units (OTUs). Closed- and open-reference OTU assignment matches reads to a reference database at 97%… read more here.
Keywords: diversity; community; closed open; open reference ... See more keywords