Measurement of Superoxide Production and NADPH Oxidase Activity by HPLC Analysis of Dihydroethidium Oxidation.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6625-7_19
Abstract: The fluorogenic probe dihydroethidium (DHE) is widely used for detecting intracellular superoxide. DHE oxidation by superoxide generates specifically the compound 2-hydroxyethidium (2-E+OH), so that 2-E+OH detection confers specificity to superoxide assessment among many other reactive… read more here.
Keywords: hplc; oxidation; dihydroethidium; superoxide ... See more keywords
Fluorometric Assessment of Sulfhydryl Oxidase Activity: Optimization by Response Surface Methodology
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Fluorescence"
DOI: 10.1007/s10895-021-02861-7
Abstract: Sulfhydryl oxidase was studied using a spectrofluorometric assay. The current protocol operates by using a combination of hemoglobin (HB) and hematin (HT) as a peroxidase mimic to catalyze the H 2 O 2 -dependent oxidation… read more here.
Keywords: oxidase activity; method; methodology; sulfhydryl oxidase ... See more keywords
Measurement of lysyl oxidase activity from small tissue samples and cell cultures.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in cell biology"
DOI: 10.1016/bs.mcb.2017.08.009
Abstract: Increasing interest in the multifunctional lysyl oxidase family of proteins is evident from the growth in the number of new publications each year. The enzymes have unique properties of strong affinities to extracellular matrix components,… read more here.
Keywords: tissue samples; samples cell; oxidase activity; lysyl oxidase ... See more keywords
The challenge of determining lysyl oxidase activity: Old methods and novel approaches.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2021.114508
Abstract: The lysyl oxidase (LOX) family of enzymes catalyze the oxidative deamination of lysine and hydroxylysine residues in collagen and elastin in the initiation step of the formation of covalent cross-linkages, an essential process for extracellular… read more here.
Keywords: challenge determining; oxidase activity; activity; lysyl oxidase ... See more keywords
Ag-doped CdO nanocatalysts: Preparation, characterization and catechol oxidase activity
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Molecular Structure"
DOI: 10.1016/j.molstruc.2018.02.031
Abstract: Abstract Silver doped cadmium oxide (Ag/CdO) nanoparticles with an average size of 41 nm have been successfully synthesized via thermal decomposition and liquid impregnation technique. The structural characterization has been performed by using several spectroscopic techniques,… read more here.
Keywords: doped cdo; oxidase activity; catechol oxidase; spectroscopy ... See more keywords
Chlorocob(II)alamin Formation Which Enhances the Thiol Oxidase Activity of the B12-Trafficking Protein CblC.
Sign Up to like & getrecommendations! Published in 2020 at "Inorganic chemistry"
DOI: 10.1021/acs.inorgchem.0c02653
Abstract: CblC is a chaperone that catalyzes removal of the β-axial ligand of cobalamin (or B12), generating cob(II)alamin in an early step in the cofactor trafficking pathway. Cob(II)alamin is subsequently partitioned to support cellular needs for… read more here.
Keywords: oxidase activity; chlorocob alamin; activity; thiol oxidase ... See more keywords
Pediatric systemic lupus erythematosus: phagocytic defect and oxidase activity of neutrophils
Sign Up to like & getrecommendations! Published in 2022 at "Pediatric Research"
DOI: 10.1038/s41390-022-02055-2
Abstract: There are limited data on neutrophil function in pediatric-onset systemic lupus erythematosus (pSLE) patients. This study aimed to evaluate phagocytosis and oxidase activity of neutrophils in patients with pSLE. Eighty-seven patients with pSLE and 44… read more here.
Keywords: oxidase activity; phagocytic activity; activity; disease activity ... See more keywords
A simple and direct ionic chromatography method to monitor galactose oxidase activity
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DOI: 10.1039/d2ra04485d
Abstract: Galactose oxidase (GalOx, EC.1.1.3.9) is one of the most extensively studied copper radical oxidases (CROs). The reaction catalyzed by GalOx leads to the oxidation of the C-6 hydroxyl group of galactose and galactosides (including galactosylated… read more here.
Keywords: galactose oxidase; simple direct; activity; method ... See more keywords
Lysyl oxidase activity in human skin is increased by chronic ultraviolet radiation exposure and smoking
Sign Up to like & getrecommendations! Published in 2017 at "British Journal of Dermatology"
DOI: 10.1111/bjd.14959
Abstract: Lifestyle choices, such as tobacco smoking and sunbathing can lead to premature skin ageing1. These extrinsic accelerators of the ageing process cause profound changes to the abundance and arrangement of the elastic fibres and fibrillar… read more here.
Keywords: increased chronic; human skin; oxidase activity; lysyl oxidase ... See more keywords