P450 BM3‐Catalyzed Regio‐ and Stereoselective Hydroxylation Aiming at the Synthesis of Phthalides and Isocoumarins
Sign Up to like & getrecommendations! Published in 2017 at "ChemBioChem"
DOI: 10.1002/cbic.201600685
Abstract: Cytochrome P450 BM3 monooxygenases are able to catalyze the regio‐ and stereoselective oxygenation of a broad range of substrates, with promising potential for synthetic applications. To study the suitability of P450 BM3 variants for stereoselective… read more here.
Keywords: p450 bm3; synthesis phthalides; hydroxylation; phthalides isocoumarins ... See more keywords
Surface charge-controlled electron transfer and catalytic behavior of immobilized cytochrome P450 BM3 inside dendritic mesoporous silica nanoparticles
Sign Up to like & getrecommendations! Published in 2020 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-020-02727-0
Abstract: Understanding the influencing factors on the reaction kinetics of P450 BM3 within confined spaces is essential for developing efficient P450 BM3 bioreactors. Herein, two dendritic mesoporous silica nanoparticles (OH-DMSNs and NH 2 -DMSNs) with similar… read more here.
Keywords: surface charge; p450 bm3; dmsns; bm3 ... See more keywords
MicroGelzymes: pH-Independent Immobilization of Cytochrome P450 BM3 in Microgels.
Sign Up to like & getrecommendations! Published in 2020 at "Biomacromolecules"
DOI: 10.1021/acs.biomac.0c01262
Abstract: Microgels are an emerging class of "ideal" enzyme carriers because of their chemical and process stability, biocompatibility, and high enzyme loading capability. In this work, we synthesized a new type of permanently positively charged poly(N-vinylcaprolactam)… read more here.
Keywords: pvcl vimq; p450 bm3; immobilization; p450 ... See more keywords
Novel insights into P450 BM3 interactions with FDA-approved antifungal azole drugs
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DOI: 10.1038/s41598-018-37330-y
Abstract: Flavocytochrome P450 BM3 is a natural fusion protein constructed of cytochrome P450 and NADPH-cytochrome P450 reductase domains. P450 BM3 binds and oxidizes several mid- to long-chain fatty acids, typically hydroxylating these lipids at the ω-1,… read more here.
Keywords: p450 bm3; azole drugs; bm3; insights p450 ... See more keywords
Engineering the biomimetic cofactors of NMNH for cytochrome P450 BM3 based on binding conformation refinement
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DOI: 10.1039/d1ra00352f
Abstract: A rational design strategy was proposed to improve the efficient utilization of alternative biomimetic cofactor by P450 BM3 enzyme. read more here.
Keywords: biomimetic cofactors; p450 bm3; engineering biomimetic; cofactors nmnh ... See more keywords
Enhanced and specific epoxidation activity of P450 BM3 mutants for the production of high value terpene derivatives
Sign Up to like & getrecommendations! Published in 2022 at "RSC Advances"
DOI: 10.1039/d2ra06029a
Abstract: Terpenes are natural molecules of valuable interest for different industrial applications. Cytochromes P450 enzymes can functionalize terpenoids to form high value oxidized derivatives in a green and sustainable manner, representing a valid alternative to chemical… read more here.
Keywords: epoxidation activity; enhanced specific; bm3; high value ... See more keywords
Directed Evolution of P450 BM3 towards Functionalization of Aromatic O-Heterocycles
Sign Up to like & getrecommendations! Published in 2019 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms20133353
Abstract: The O-heterocycles, benzo-1,4-dioxane, phthalan, isochroman, 2,3-dihydrobenzofuran, benzofuran, and dibenzofuran are important building blocks with considerable medical application for the production of pharmaceuticals. Cytochrome P450 monooxygenase (P450) Bacillus megaterium 3 (BM3) wild type (WT) from Bacillus… read more here.
Keywords: evolution p450; p450 bm3; bm3; directed evolution ... See more keywords