Exploring PTDH–P450BM3 Variants for the Synthesis of Drug Metabolites
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DOI: 10.1002/cbic.201700470
Abstract: The conversion of a series of pharmaceutical compounds was examined with three variants of cytochrome P450BM3 fused to phosphite dehydrogenase (PTDH) to enable cofactor recycling. Conditions for enzyme production were optimized, and the purified PTDH–P450BM3… read more here.
Keywords: p450bm3; ptdh p450bm3; p450bm3 variants; variants synthesis ... See more keywords
Stabilization of the Reductase Domain in the Catalytically Self‐Sufficient Cytochrome P450BM3 by Consensus‐Guided Mutagenesis
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DOI: 10.1002/cbic.201700546
Abstract: The multidomain, catalytically self‐sufficient cytochrome P450 BM‐3 from Bacillus megaterium (P450BM3) constitutes a versatile enzyme for the oxyfunctionalization of organic molecules and natural products. However, the limited stability of the diflavin reductase domain limits the… read more here.
Keywords: p450bm3; mutagenesis; reductase; consensus ... See more keywords