Genome‐wide detection of ultralow‐frequency substitution mutations in cultures of mouse lymphoma L5178Y cells and Caenorhabditis elegans worms by PacBio sequencing
Sign Up to like & getrecommendations! Published in 2022 at "Environmental and Molecular Mutagenesis"
DOI: 10.1002/em.22473
Abstract: Many conventional genetic toxicology assays require specialized cell cultures or animals and can only detect mutations that inactivate the function of a reporter gene. These limitations make such assays incompatible with many toxicological models but… read more here.
Keywords: elegans worms; pacbio sequencing; genome wide; detection ... See more keywords
Analysis of recombinational switching at the antigenic variation locus of the Lyme spirochete using a novel PacBio sequencing pipeline
Sign Up to like & getrecommendations! Published in 2018 at "Molecular Microbiology"
DOI: 10.1111/mmi.13873
Abstract: The Lyme disease spirochete evades the host immune system by combinatorial variation of VlsE, a surface antigen. Antigenic variation occurs via segmental gene conversion from contiguous silent cassettes into the vlsE locus. Because of the… read more here.
Keywords: pacbio sequencing; variation; antigenic variation; pipeline ... See more keywords
PaSS: a sequencing simulator for PacBio sequencing
Sign Up to like & getrecommendations! Published in 2019 at "BMC Bioinformatics"
DOI: 10.1186/s12859-019-2901-7
Abstract: BackgroundThird-generation sequencing platforms, such as PacBio sequencing, have been developed rapidly in recent years. PacBio sequencing generates much longer reads than the second-generation sequencing (or the next generation sequencing, NGS) technologies and it has unique… read more here.
Keywords: pacbio sequencing; sequencing data; simulator; pass ... See more keywords