A novel mutation tolerant padlock probe design for multiplexed detection of hypervariable RNA viruses
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-39854-3
Abstract: The establishment of a robust detection platform for RNA viruses still remains a challenge in molecular diagnostics due to their high mutation rates. Newcastle disease virus (NDV) is one such RNA avian virus with a… read more here.
Keywords: detection; rna viruses; design; rna ... See more keywords
A Novel Rolling Circle Amplification-Based Detection of SARS-CoV-2 with Multi-Region Padlock Hybridization
Sign Up to like & getrecommendations! Published in 2022 at "Diagnostics"
DOI: 10.3390/diagnostics12092252
Abstract: SARS-CoV-2 has remained a global health burden, primarily due to the continuous evolution of different mutant strains. These mutations present challenges to the detection of the virus, as the target genes of qPCR, the standard… read more here.
Keywords: rolling circle; circle amplification; detection sars; detection ... See more keywords
Effects of Microchannel Shape and Ultrasonic Mixing on Microfluidic Padlock Probe Rolling Circle Amplification (RCA) Reactions
Sign Up to like & getrecommendations! Published in 2018 at "Micromachines"
DOI: 10.3390/mi9060272
Abstract: The fluorescence in situ hybridization (FISH)-based padlock probe and rolling circle amplification (RCA) method allows for the detection of point mutations. However, it requires multiple reaction steps and solution exchanges, making it costly, labor-intensive, and… read more here.
Keywords: padlock probe; probe rolling; rolling circle; rca ... See more keywords