Efficient in situ barcode sequencing using padlock probe-based BaristaSeq
Sign Up to like & getrecommendations! Published in 2018 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkx1206
Abstract: Abstract Cellular DNA/RNA tags (barcodes) allow for multiplexed cell lineage tracing and neuronal projection mapping with cellular resolution. Conventional approaches to reading out cellular barcodes trade off spatial resolution with throughput. Bulk sequencing achieves high… read more here.
Keywords: resolution; padlock probe; barcode sequencing; probe based ... See more keywords
Padlock Probe-Based Targeted In Situ Sequencing: Overview of Methods and Applications.
Sign Up to like & getrecommendations! Published in 2023 at "Annual review of genomics and human genetics"
DOI: 10.1146/annurev-genom-102722-092013
Abstract: Elucidating spatiotemporal changes in gene expression has been an essential goal in studies of health, development, and disease. In the emerging field of spatially resolved transcriptomics, gene expression profiles are acquired with the tissue architecture… read more here.
Keywords: genetics; probe based; situ sequencing; padlock probe ... See more keywords
Simple Detection of DNA Methyltransferase with an Integrated Padlock Probe
Sign Up to like & getrecommendations! Published in 2022 at "Biosensors"
DOI: 10.3390/bios12080569
Abstract: DNA methyltransferases (MTases) can be regarded as biomarkers, as demonstrated by many studies on genetic diseases. Many researchers have developed biosensors to detect the activity of DNA MTases, and nucleic acid amplification, which need other… read more here.
Keywords: dna; detection; dna mtases; padlock probe ... See more keywords
Effects of Microchannel Shape and Ultrasonic Mixing on Microfluidic Padlock Probe Rolling Circle Amplification (RCA) Reactions
Sign Up to like & getrecommendations! Published in 2018 at "Micromachines"
DOI: 10.3390/mi9060272
Abstract: The fluorescence in situ hybridization (FISH)-based padlock probe and rolling circle amplification (RCA) method allows for the detection of point mutations. However, it requires multiple reaction steps and solution exchanges, making it costly, labor-intensive, and… read more here.
Keywords: padlock probe; probe rolling; rolling circle; rca ... See more keywords