Paired Design of dCas9 as a Systematic Platform for the Detection of Featured Nucleic Acid Sequences in Pathogenic Strains.
Sign Up to like & getrecommendations! Published in 2017 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.6b00215
Abstract: We developed an in vitro DNA detection system using a pair of dCas9 proteins linked to split halves of luciferase. Luminescence was induced upon colocalization of the reporter pair to a ∼44 bp target sequence… read more here.
Keywords: detection; design dcas9; paired design; dcas9 systematic ... See more keywords
Application of Weighted Gene Co-expression Network Analysis for Data from Paired Design
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-18705-z
Abstract: Investigating how genes jointly affect complex human diseases is important, yet challenging. The network approach (e.g., weighted gene co-expression network analysis (WGCNA)) is a powerful tool. However, genomic data usually contain substantial batch effects, which… read more here.
Keywords: weighted gene; network; paired design; gene expression ... See more keywords