Selective loading and processing of prespacers for precise CRISPR adaptation
Sign Up to like & getrecommendations! Published in 2020 at "Nature"
DOI: 10.1038/s41586-020-2018-1
Abstract: CRISPR–Cas immunity protects prokaryotes against invading genetic elements 1 . It uses the highly conserved Cas1–Cas2 complex to establish inheritable memory (spacers) 2 – 5 . How Cas1–Cas2 acquires spacers from foreign DNA fragments (prespacers)… read more here.
Keywords: pam sequence; correct orientation; integration; cas1 cas2 ... See more keywords
CRISPRactivation-SMS, a message for PAM sequence independent gene up-regulation in Escherichia coli
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac804
Abstract: Abstract Governance of the endogenous gene regulatory network enables the navigation of cells towards beneficial traits for recombinant protein production. CRISPRactivation and interference provides the basis for gene expression modulation but is primarily applied in… read more here.
Keywords: escherichia coli; crispra; pam sequence; regulation ... See more keywords