MicroRNA In Situ Hybridization in Paraffin-Embedded Cultured Cells.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0623-0_6
Abstract: MicroRNA-21 (miR-21) is one of the most abundant microRNAs in cancer tissues and is considered a strong prognostic biomarker. In situ hybridization (ISH) analyses using locked nucleic acid (LNA) probes have shown that miR-21 is… read more here.
Keywords: cultured cells; paraffin embedded; hybridization; cancer ... See more keywords
Kupffer Cell and Monocyte-Derived Macrophage Identification by Immunofluorescence on Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse Liver Sections.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0704-6_6
Abstract: Kupffer cells are the liver-resident macrophages and represent the first line of defense between the pathogens circulating from the intestines through the portal vein and systemic circulation. Recent works have highlighted the complex heterogeneity of… read more here.
Keywords: fixed paraffin; paraffin embedded; formalin fixed; macrophage ... See more keywords
Round robin study of formalin-fixed paraffin-embedded tissues in mass spectrometry imaging
Sign Up to like & getrecommendations! Published in 2018 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-018-1216-2
Abstract: AbstractMass spectrometry imaging (MSI) has provided many results with translational character, which still have to be proven robust in large patient cohorts and across different centers. Although formalin-fixed paraffin-embedded (FFPE) specimens are most common in… read more here.
Keywords: fixed paraffin; msi; paraffin embedded; study ... See more keywords
Cloning of Helicobacter suis cholesterol α-glucosyltransferase and production of an antibody capable of detecting it in formalin-fixed, paraffin-embedded gastric tissue sections
Sign Up to like & getrecommendations! Published in 2017 at "Histochemistry and Cell Biology"
DOI: 10.1007/s00418-017-1582-4
Abstract: Helicobacter suis (H. suis), formerly called Helicobacter heilmannii type 1 (H. heilmannii), is a gram-negative bacterium of the Helicobacter species. This pathogen infects the stomach of humans and animals such as dogs, cats, pigs, and… read more here.
Keywords: fixed paraffin; paraffin embedded; antibody; helicobacter ... See more keywords
Enhanced phylogenetic resolution of Newcastle disease outbreaks using complete viral genome sequences from formalin-fixed paraffin-embedded tissue samples
Sign Up to like & getrecommendations! Published in 2019 at "Virus Genes"
DOI: 10.1007/s11262-019-01669-9
Abstract: Highly virulent Newcastle disease virus (NDV) causes Newcastle disease (ND), which is a threat to poultry production worldwide. Effective disease management requires approaches to accurately determine sources of infection, which involves tracking of closely related… read more here.
Keywords: disease outbreaks; fixed paraffin; paraffin embedded; disease ... See more keywords
Evaluation of Mutations in 23S rRNA, rdxA and frxA Genes of Helicobacter pylori in Paraffin-Embedded Gastric Biopsy Specimens from Iranian Gastric Cancer and Gastritis Patients
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Gastrointestinal Cancer"
DOI: 10.1007/s12029-020-00386-z
Abstract: Purpose Helicobacter pylori (H. pylori) infection is considered as one of the main cause of gastric cancer. Treatment failure of the infection often occurs due to antibiotic resistance. Herein, we aimed to evaluate the mutations… read more here.
Keywords: rdxa frxa; frxa genes; gastric cancer; paraffin embedded ... See more keywords
Reciprocal nerve staining (RNS) for the concurrent detection of choline acetyltransferase and myelin basic protein on paraffin-embedded sections
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Neuroscience Methods"
DOI: 10.1016/j.jneumeth.2018.10.040
Abstract: BACKGROUND Objective of our work was to develop a sequential double nonfluorescent immunostaining method which allows the selective identification of myelinated motor fibers in paraffin-embedded samples of peripheral nerves. Motor recovery after a nerve gap-lesion… read more here.
Keywords: paraffin embedded; myelin basic; choline acetyltransferase; basic protein ... See more keywords
Evaluation of a panel of antibodies for the immunohistochemical identification of immune cells in paraffin-embedded lymphoid tissues of new- and old-world camelids
Sign Up to like & getrecommendations! Published in 2017 at "Veterinary Immunology and Immunopathology"
DOI: 10.1016/j.vetimm.2017.01.001
Abstract: Abstract Different species of camelids play an important role in the epidemiology of various emerging infectious diseases such as Middle East respiratory syndrome. For precise investigations of the immunopathogenesis in these host species, appropriate immunohistochemical… read more here.
Keywords: immune; panel antibodies; paraffin embedded; evaluation panel ... See more keywords
Complete genome reconstruction and genetic analysis of Taura syndrome virus of shrimp from archival Davidson's-fixed paraffin embedded tissue.
Sign Up to like & getrecommendations! Published in 2020 at "Virology"
DOI: 10.1016/j.virol.2020.11.009
Abstract: Davidson's-fixed paraffin-embedded (DFPE) shrimp tissue are a priceless biological resource for pathogen discovery and evolutionary studies for aquaculture disease diagnostic laboratories worldwide. Nucleic acids extracted from DFPE tissues are often not adequate for most downstream… read more here.
Keywords: fixed paraffin; paraffin embedded; tissue; virus ... See more keywords
Laser capture microdissection on formalin-fixed and paraffin-embedded renal transplanted biopsies: Technical perspectives for clinical practice application.
Sign Up to like & getrecommendations! Published in 2020 at "Experimental and molecular pathology"
DOI: 10.1016/j.yexmp.2020.104516
Abstract: Renal biopsy (RBx) is an essential tool in the diagnostic and therapeutic process of most native kidney diseases and in the renal transplanted graft. Laser capture microdissection (LCM), combined with molecular biology, might improve the… read more here.
Keywords: laser capture; fixed paraffin; paraffin embedded; renal transplanted ... See more keywords
In-Depth Mass Spectrometry-Based Proteomics of Formalin-Fixed, Paraffin-Embedded Tissues with a Spatial Resolution of 50-200 μm.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.2c00409
Abstract: Formalin-fixed, paraffin-embedded (FFPE) tissues are banked in large repositories to cost-effectively preserve valuable specimens for later study. With the rapid growth of spatial proteomics, FFPE tissues can serve as a more accessible alternative to more… read more here.
Keywords: formalin fixed; ffpe tissues; resolution; paraffin embedded ... See more keywords