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Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7201-2_4
Abstract: With the advent of new methodologies, proteomics-based assays are increasingly used to study the efficacy of drugs on a molecular level. For these studies to be meaningful, the proteomics assays need to be sensitive, selective,…
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Keywords:
parallel reaction;
modifying enzyme;
reaction monitoring;
prm ... See more keywords
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Published in 2019 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-019-01775-5
Abstract: AbstractThe N-glycosylation of proteins is one of the most important post-translational modifications relevant to various biological functions. The identification and quantification of N-glycoproteins in liquid chromatography–mass spectrometry (LC-MS) is challenging because of their low analytical…
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Keywords:
hepatocellular carcinoma;
reaction monitoring;
parallel reaction;
multiplex immunoprecipitation ... See more keywords
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Published in 2020 at "Energy Conversion and Management"
DOI: 10.1016/j.enconman.2020.112531
Abstract: Abstract Biomass is a renewable source and is considered as an alternative to fossil fuels. Bioenergy can be released from biomass combustion while various bioproducts can be produced through gasification and pyrolysis. Among these three…
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Keywords:
parallel reaction;
pyrolysis;
biomass;
model ... See more keywords
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Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c01375
Abstract: Mass spectrometry-based targeted proteomics employs heavy isotope-labeled proteins or peptides as standards to improve accuracy and precision. The input sample amount is often determined by the total quantity of endogenous proteins or peptides, as defined…
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Keywords:
parallel reaction;
reaction monitoring;
targeted proteomics;
global extraction ... See more keywords
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Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c03432
Abstract: Here, we have documented a new protocol to determine d/l-amino acids by derivatizing amino acids via a chiral phosphinate. (RP)-l-Menthyl phenylphosphinate was able to bond both primary and secondary amines, as well as improve the…
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Keywords:
amino acids;
parallel reaction;
reaction monitoring;
sensitivity ... See more keywords
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Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c05770
Abstract: The outbreak of coronavirus disease 2019 (COVID-19) has overwhelmed the global economy and human well-being. On account of the sharp increase in test demand, there is a need for an accurate and alternative diagnosis method…
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Keywords:
parallel reaction;
high sensitivity;
detection;
cov glycoprotein ... See more keywords
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Published in 2017 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.7b02283
Abstract: Acylcarnitines are exerting a variety of biological functions depending on the differences in lengths, saturation levels, and conjugation groups, which to a great extent contribute to the challenges of acylcarnitines quantifications due to various kinds…
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Keywords:
high resolution;
resolution parallel;
novel method;
parallel reaction ... See more keywords
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Published in 2021 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.1c00377
Abstract: Parallel reaction monitoring (PRM) is an increasingly popular alternative to selected reaction monitoring (SRM) for targeted proteomics. PRM's strengths over SRM are that it monitors all product ions in a single spectrum, thus eliminating the…
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Keywords:
resolution;
high resolution;
parallel reaction;
reaction monitoring ... See more keywords
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Published in 2021 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.1c05295
Abstract: Structural characterization of glycerophospholipids beyond the fatty acid level has become a major endeavor in lipidomics, presenting an opportunity to advance the understanding of the intricate relationship between lipid metabolism and disease state. Distinguishing subtle…
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Keywords:
parallel reaction;
mass spectrometry;
quantitation;
mass ... See more keywords
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Published in 2018 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.1811059115
Abstract: Significance Thymidylate synthase (TSase) is one of the prime anticancer targets, as it generates the sole de novo source of thymidylate, a unique precursor for DNA. Fluoropyrimidines and antifolates inhibiting TSase pathways are not only…
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Keywords:
thymidylate synthase;
parallel reaction;
noncovalent intermediates;
mechanics ... See more keywords
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Published in 2022 at "PLoS ONE"
DOI: 10.1371/journal.pone.0271057
Abstract: Bovine pregnancy-associated glycoproteins (boPAGs) are extensively glycosylated secretory proteins of trophoblast cells. Roughly 20 different boPAG members are known but their distribution patterns and degree of glycosylation during pregnancy are not well characterized. The objective…
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Keywords:
parallel reaction;
glycosylation;
pregnancy;
bovine pregnancy ... See more keywords