Dissection of c-AMP Response Element Architecture by Using Genomic and Episomal Massively Parallel Reporter Assays.
Sign Up to like & getrecommendations! Published in 2020 at "Cell systems"
DOI: 10.1016/j.cels.2020.05.011
Abstract: In eukaryotes, transcription factors (TFs) orchestrate gene expression by binding to TF-binding sites (TFBSs) and localizing transcriptional co-regulators and RNA polymerase II to cis-regulatory elements. However, we lack a basic understanding of the relationship between… read more here.
Keywords: massively parallel; amp response; expression; parallel reporter ... See more keywords
Deep learning-assisted genome-wide characterization of massively parallel reporter assays
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac990
Abstract: Abstract Massively parallel reporter assay (MPRA) is a high-throughput method that enables the study of the regulatory activities of tens of thousands of DNA oligonucleotides in a single experiment. While MPRA experiments have grown in… read more here.
Keywords: mpra; genome wide; parallel reporter; massively parallel ... See more keywords
Linear models enable powerful differential activity analysis in massively parallel reporter assays
Sign Up to like & getrecommendations! Published in 2019 at "BMC Genomics"
DOI: 10.1186/s12864-019-5556-x
Abstract: BackgroundMassively parallel reporter assays (MPRAs) have emerged as a popular means for understanding noncoding variation in a variety of conditions. While a large number of experiments have been described in the literature, analysis typically uses… read more here.
Keywords: analysis; reporter assays; parallel reporter; models enable ... See more keywords
MPRAnalyze: statistical framework for massively parallel reporter assays
Sign Up to like & getrecommendations! Published in 2019 at "Genome Biology"
DOI: 10.1186/s13059-019-1787-z
Abstract: Massively parallel reporter assays (MPRAs) can measure the regulatory function of thousands of DNA sequences in a single experiment. Despite growing popularity, MPRA studies are limited by a lack of a unified framework for analyzing… read more here.
Keywords: framework; massively parallel; reporter assays; parallel reporter ... See more keywords
Characterization of De Novo Promoter Variants in Autism Spectrum Disorder with Massively Parallel Reporter Assays
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24043509
Abstract: Autism spectrum disorder (ASD) is a common, complex, and highly heritable condition with contributions from both common and rare genetic variations. While disruptive, rare variants in protein-coding regions clearly contribute to symptoms, the role of… read more here.
Keywords: autism spectrum; parallel reporter; spectrum disorder; reporter assays ... See more keywords