Massively parallel sequencing techniques for forensics: A review
Sign Up to like & getrecommendations! Published in 2018 at "Electrophoresis"
DOI: 10.1002/elps.201800082
Abstract: DNA sequencing, starting with Sanger's chain termination method in 1977 and evolving into the next generation sequencing (NGS) techniques of today that employ massively parallel sequencing (MPS), has become essential in application areas such as… read more here.
Keywords: dna; massively parallel; sequencing techniques; forensics review ... See more keywords
Isometric artifacts from polymerase chain reaction‐massively parallel sequencing analysis of short tandem repeat loci: An emerging issue from a new technology?
Sign Up to like & getrecommendations! Published in 2022 at "Electrophoresis"
DOI: 10.1002/elps.202100143
Abstract: The recent introduction of polymerase chain reaction (PCR)‐massively parallel sequencing (MPS) technologies in forensics has changed the approach to allelic short tandem repeat (STR) typing because sequencing cloned PCR fragments enables alleles with identical molecular… read more here.
Keywords: chain reaction; polymerase chain; parallel sequencing; massively parallel ... See more keywords
Impact of DNA degradation on massively parallel sequencing-based autosomal STR, iiSNP, and mitochondrial DNA typing systems
Sign Up to like & getrecommendations! Published in 2019 at "International Journal of Legal Medicine"
DOI: 10.1007/s00414-019-02110-4
Abstract: Biological samples, including skeletal remains exposed to environmental insults for extended periods of time, exhibit increasing levels of DNA damage and fragmentation. Human forensic identification methods typically use a combination of mitochondrial (mt) DNA sequencing… read more here.
Keywords: dna; massively parallel; parallel sequencing; skeletal remains ... See more keywords
Sudden unexpected death in the young - Value of massive parallel sequencing in postmortem genetic analyses.
Sign Up to like & getrecommendations! Published in 2018 at "Forensic science international"
DOI: 10.1016/j.forsciint.2018.09.034
Abstract: Cases of sudden cardiac death (SCD) in young and apparently healthy individuals represent a devastating event in affected families. Hereditary arrhythmia syndromes, which include primary electrical heart disorders as well as cardiomyopathies, are known to… read more here.
Keywords: death; postmortem genetic; genetic analyses; parallel sequencing ... See more keywords
Parallel sequencing of 60 X-chromosome genetic markers including STRs, SNPs and InDels
Sign Up to like & getrecommendations! Published in 2017 at "Forensic Science International: Genetics Supplement Series"
DOI: 10.1016/j.fsigss.2017.09.127
Abstract: Abstract Massively parallel sequencing (MPS) technology has become popular in forensic studies, due to the general gains of larger multiplexing and the detection of sequence variation. In this study, we present the sequencing results from… read more here.
Keywords: snps indels; snps; genetics; strs snps ... See more keywords
Massively parallel sequencing of 24 Y-STR loci in Thai population
Sign Up to like & getrecommendations! Published in 2017 at "Forensic Science International: Genetics Supplement Series"
DOI: 10.1016/j.fsigss.2017.09.129
Abstract: Abstract Massively parallel sequencing technology has been demonstrated as a potential tool for genetic studies and recently, for forensic context. ForenSeq™ DNA Signature Prep Kit (Illumina, Inc.) has been designed exclusively for forensic DNA analysis… read more here.
Keywords: loci; massively parallel; thai population; str loci ... See more keywords
Molecular profiling of vitreous fluid by massively parallel sequencing: a case series.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of the American Society of Cytopathology"
DOI: 10.1016/j.jasc.2020.03.005
Abstract: INTRODUCTION In cases of suspected intraocular malignancy, vitreous may be the preferred pathologic sample; however, cellularity may be insufficient for definitive cytopathological diagnosis. Ancillary methodology to study vitreous fluid aspiration for mutational analysis may assist… read more here.
Keywords: massively parallel; molecular profiling; case; vitreous fluid ... See more keywords
Massively parallel sequencing and targeted exomes in familial kidney disease can diagnose underlying genetic disorders.
Sign Up to like & getrecommendations! Published in 2017 at "Kidney international"
DOI: 10.1016/j.kint.2017.06.013
Abstract: Inherited kidney disease encompasses a broad range of disorders, with both multiple genes contributing to specific phenotypes and single gene defects having multiple clinical presentations. Advances in sequencing capacity may allow a genetic diagnosis for… read more here.
Keywords: massively parallel; disease; parallel sequencing; diagnose underlying ... See more keywords
37. PREIMPLANTATION GENETIC TESTING: TO THE OPEN QUESTIONS ABOUT DATA INTERPRETATION OBTAINED BY MASSIVE PARALLEL SEQUENCING
Sign Up to like & getrecommendations! Published in 2019 at "Reproductive BioMedicine Online"
DOI: 10.1016/j.rbmo.2019.04.090
Abstract: Introduction the accuracy results of preimplantation genetic testing of embryos for aneuploidy (PGT-A) by massive parallel sequencing directly depends on the effectiveness of the quality control. The aim of this study was to analyze the… read more here.
Keywords: quality; genetic testing; parallel sequencing; pgt ... See more keywords
Massively Parallel Sequencing (MPS) of Cell-Free Fetal DNA (cffDNA) for Trisomies 21, 18, and 13 in Twin Pregnancies
Sign Up to like & getrecommendations! Published in 2017 at "Twin Research and Human Genetics"
DOI: 10.1017/thg.2017.23
Abstract: Massively parallel sequencing (MPS) technology has become increasingly available and has been widely used to screen for trisomies 21, 18, and 13 in singleton pregnancies. This study assessed the performance of MPS testing of cell-free… read more here.
Keywords: sequencing mps; massively parallel; cell free; parallel sequencing ... See more keywords
Targeted massively parallel sequencing of mature lymphoid neoplasms: assessment of empirical application and diagnostic utility in routine clinical practice
Sign Up to like & getrecommendations! Published in 2020 at "Modern Pathology"
DOI: 10.1038/s41379-020-00720-7
Abstract: Massively parallel sequencing (MPS) has become a viable diagnostic tool to interrogate genetic profiles of numerous tumors but has yet to be routinely adopted in the setting of lymphoma. Here, we report the empirical application… read more here.
Keywords: massively parallel; empirical application; lymphoid neoplasms; application ... See more keywords