Transient Transformation Using Particle Bombardment for Gene Expression Analysis.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8778-8_5
Abstract: Transient transformation or transient expression results in rapid and fleeting gene expression. This approach is often used as a first-tier screening tool for evaluation of components that affect gene expression. Here, we describe the use… read more here.
Keywords: transient transformation; particle bombardment; expression; gene expression ... See more keywords
Gene Expression in Citrus Plant Cells Using Helios® Gene Gun System for Particle Bombardment.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9558-5_16
Abstract: To understand how Citrus tristeza virus (CTV) replicates and moves inside the plant, it is critical to study the cellular interactions and localization of its encoded proteins. However, due to technical limitations, so far these… read more here.
Keywords: plant; particle bombardment; helios gene; citrus ... See more keywords
Factors affecting genetic transformation by particle bombardment of the prickly pear cactus (O. ficus-indica)
Sign Up to like & getrecommendations! Published in 2019 at "3 Biotech"
DOI: 10.1007/s13205-019-1627-6
Abstract: In the present study, a novel transformation protocol for Opuntia ficus-indica was generated by means of particle bombardment. The best conditions obtained were: 900 psi rupture disk pressure, 8 cm microprojectile travel distance, and 4 h of… read more here.
Keywords: affecting genetic; particle bombardment; factors affecting; transformation ... See more keywords
An established protocol for generating transgenic wheat for wheat functional genomics via particle bombardment
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2022.979540
Abstract: Wheat is one of the most important food crops in the world and is considered one of the top targets in crop biotechnology. With the high-quality reference genomes of wheat and its relative species and… read more here.
Keywords: wheat; transgenic wheat; particle bombardment; bombardment mediated ... See more keywords
In planta particle bombardment (iPB): A new method for plant transformation and genome editing.
Sign Up to like & getrecommendations! Published in 2020 at "Plant biotechnology"
DOI: 10.5511/plantbiotechnology.20.0206a
Abstract: Transformation is a key step in modern breeding technology that involves genome editing. The requirement for in vitro tissue culture and regeneration hampers application of this technology to commercially important varieties of many crop species.… read more here.
Keywords: genome editing; planta particle; plant; particle bombardment ... See more keywords
Particle bombardment and subcellular protein localization analysis in the aquatic plant Egeria densa
Sign Up to like & getrecommendations! Published in 2017 at "PeerJ"
DOI: 10.7717/peerj.3779
Abstract: Particle bombardment is a powerful and relatively easy method for transient expression of genes of interest in plant cells, especially those that are recalcitrant to other transformation methods. This method has facilitated numerous analyses of… read more here.
Keywords: particle bombardment; localization; localization analysis; plant ... See more keywords