Determination of the ribosome structure to a resolution of 2.5 Å by single‐particle cryo‐EM
Sign Up to like & getrecommendations! Published in 2017 at "Protein Science"
DOI: 10.1002/pro.3068
Abstract: With the advance of new instruments and algorithms, and the accumulation of experience over decades, single‐particle cryo‐EM has become a pivotal part of structural biology. Recently, we determined the structure of a eukaryotic ribosome at… read more here.
Keywords: ribosome; resolution; single particle; particle cryo ... See more keywords
Single-particle cryo-EM studies of transmembrane proteins in SMA copolymer nanodiscs.
Sign Up to like & getrecommendations! Published in 2019 at "Chemistry and physics of lipids"
DOI: 10.1016/j.chemphyslip.2019.03.007
Abstract: Styrene-maleic acid (SMA) copolymers can extract membrane proteins from native membranes along with lipids as nanodiscs. Preparation with SMA is fast, cost-effective, and captures the native protein-lipid interactions. On the other hand, cryo-EM has become… read more here.
Keywords: membrane proteins; studies transmembrane; particle cryo; single particle ... See more keywords
Could Egg White Lysozyme be Solved by Single Particle Cryo-EM?
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Chemical Information and Modeling"
DOI: 10.1021/acs.jcim.9b01176
Abstract: The combination of high-end cryogenic transmission electron microscopes (cryo-EM), direct electron detectors, and advanced image algorithms allows researchers to obtain the 3D structures of much smaller macromolecules than years ago. However, there are still major… read more here.
Keywords: particle; particle cryo; white lysozyme; egg white ... See more keywords
Single-particle cryo-EM using alignment by classification (ABC): the structure of Lumbricus terrestris haemoglobin
Sign Up to like & getrecommendations! Published in 2017 at "IUCrJ"
DOI: 10.1107/s2052252517010922
Abstract: An efficient and fast pipeline is presented for obtaining near-atomic resolution structures from large single-particle cryo-EM data sets. The approach is virtually reference-free and is therefore less prone to the perils of reference bias. read more here.
Keywords: using alignment; cryo using; single particle; particle cryo ... See more keywords
Towards automating single-particle cryo-EM data acquisition
Sign Up to like & getrecommendations! Published in 2023 at "IUCrJ"
DOI: 10.1107/s2052252522012039
Abstract: Target selection for single-particle cryo-EM data acquisition sessions is mostly done manually by human operators, which is time consuming and leads to the inefficient use of instruments. The software toolbox Ptolemy [Kim et al. (2023).… read more here.
Keywords: data acquisition; cryo data; cryo; single particle ... See more keywords
Recent Technical Advances in Sample Preparation for Single-Particle Cryo-EM
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2022.892459
Abstract: Cryo-sample preparation is a vital step in the process of obtaining high-resolution structures of macromolecules by using the single-particle cryo–electron microscopy (cryo-EM) method; however, cryo-sample preparation is commonly hampered by high uncertainty and low reproducibility.… read more here.
Keywords: single particle; sample preparation; preparation; particle cryo ... See more keywords