Structure and function of cytoplasmic serine hydroxymethyltransferase from Pichia pastoris.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2018.01.084
Abstract: Serine hydroxymethyltransferase (SHMT) catalyzes the interconversion of serine and glycine, which is crucial for one carbon metabolism. Here, we report the first crystal structure of cytoplasmic SHMT from Pichia pastoris (pcSHMT) diffracted to 2.5 Å resolution… read more here.
Keywords: serine hydroxymethyltransferase; pichia pastoris; pastoris; structure ... See more keywords
Production of recombinant lipase B from Candida antarctica in Pichia pastoris under control of the promoter PGK using crude glycerol from biodiesel production as carbon source
Sign Up to like & getrecommendations! Published in 2017 at "Biochemical Engineering Journal"
DOI: 10.1016/j.bej.2016.11.018
Abstract: Abstract The use of new constitutive promoters and recycled carbon sources in the recombinant production of industrial proteins such as lipases in the cell factory Pichia pastoris is desirable, in order to improve production and… read more here.
Keywords: carbon; production; pastoris; using crude ... See more keywords
Towards systems metabolic engineering in Pichia pastoris.
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology advances"
DOI: 10.1016/j.biotechadv.2017.07.009
Abstract: The methylotrophic yeast Pichia pastoris is firmly established as a host for the production of recombinant proteins, frequently outperforming other heterologous hosts. Already, a sizeable amount of systems biology knowledge has been acquired for this… read more here.
Keywords: engineering; systems metabolic; pastoris; metabolic engineering ... See more keywords
Combined strategies for improving production of a thermo-alkali stable laccase in Pichia pastoris ☆
Sign Up to like & getrecommendations! Published in 2017 at "Electronic Journal of Biotechnology"
DOI: 10.1016/j.ejbt.2017.04.002
Abstract: Background: Laccases are copper-containing enzymes which have been used as green biocatalysts for many industrial processes. Although bacterial laccases have high stabilities which facilitate their application under harsh conditions, their activities and production yields are… read more here.
Keywords: pastoris; mso; thermo alkali; laccase ... See more keywords
Construction, expression and characterization of a fusion protein HBscFv-IFNγ in Komagatella (Pichia) pastoris X33.
Sign Up to like & getrecommendations! Published in 2017 at "Enzyme and microbial technology"
DOI: 10.1016/j.enzmictec.2017.04.001
Abstract: HBscFv-IFNγ, a fusion protein constructed by fusing γ-interferon (IFNγ) with an antibody fragment HBscFv for the purpose of targeted delivery of the cytokine IFNγ, was designed in order to enhance its therapeutic efficacy through increasing… read more here.
Keywords: fusion protein; hbscfv ifn; ifn; pastoris ... See more keywords
High efficiency CRISPR/Cas9 genome editing system with an eliminable episomal sgRNA plasmid in Pichia pastoris.
Sign Up to like & getrecommendations! Published in 2020 at "Enzyme and microbial technology"
DOI: 10.1016/j.enzmictec.2020.109556
Abstract: Pichia pastoris is a methylotrophic yeast in which host heterologous expression of proteins has been developed owing to the strong inducible alcohol oxidase promoter (PAOX1). However, it is difficult to manipulate the genome in P.… read more here.
Keywords: genome editing; system; pastoris; crispr cas9 ... See more keywords
Synergistic codon optimization and bioreactor cultivation toward enhanced secretion of fungal lignin peroxidase in Pichia pastoris: Enzymatic valorization of technical (industrial) lignins.
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DOI: 10.1016/j.enzmictec.2020.109593
Abstract: Lignin peroxidase (LiP) is a well-recognized enzyme for its ability to oxidize lignins, but its commercial availability is limited, which hinders the biotechnological application of LiP-based bioprocesses in lignocellulose biorefineries. This study evaluated a combination… read more here.
Keywords: lignin peroxidase; pastoris; lignin; optimization ... See more keywords
Efficient production of human goose-type lysozyme 2 in the methylotrophic yeast Pichia pastoris.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of biotechnology"
DOI: 10.1016/j.jbiotec.2018.04.010
Abstract: Infectious diseases caused by antibiotic multidrug-resistant microorganisms are major causes of morbidity and mortality in humans. Hence, there is an urgent need to search for new antimicrobial agents. Initially known as a defensive effector in… read more here.
Keywords: human goose; production; pastoris; goose type ... See more keywords
Expression of buffalo chymosin in Pichia pastoris for application in mozzarella cheese
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DOI: 10.1016/j.lwt.2017.06.033
Abstract: Abstract Chymosin, a major component of rennet, is used extensively as a milk clotting enzyme in cheese making industry. Increased demand coupled with scarce availability of native animal rennet has encouraged use of recombinant chymosin… read more here.
Keywords: chymosin; pastoris; mozzarella cheese; buffalo ... See more keywords
Agonist binding of human mu opioid receptors expressed in the yeast Pichia pastoris: Effect of cholesterol complementation
Sign Up to like & getrecommendations! Published in 2020 at "Neurochemistry International"
DOI: 10.1016/j.neuint.2019.104588
Abstract: This study compared pharmacological profiles between human mu opioid receptors (hMOR) overexpressed in the SH-SY5Y neuroblastoma cell line (SH-hMOR) and the methylotrophic yeast Pichia pastoris (Pp-hMOR). Affinity determinations were performed by direct binding with the… read more here.
Keywords: pastoris; cholesterol; human opioid; yeast pichia ... See more keywords
Auto-induction of Pichia pastoris AOX1 promoter for membrane protein expression.
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DOI: 10.1016/j.pep.2017.06.006
Abstract: Pichia pastoris is a highly successful recombinant protein expression system due to its ability to quickly generate large quantities of recombinant proteins in simple media. P. pastoris has been used to successfully generate milligram quantities of… read more here.
Keywords: protein expression; pastoris; expression; aox1 promoter ... See more keywords