Deep Learning‐Based Ion Channel Kinetics Analysis for Automated Patch Clamp Recording
Sign Up to like & getrecommendations! Published in 2024 at "Advanced Science"
DOI: 10.1002/advs.202404166
Abstract: The patch clamp technique is a fundamental tool for investigating ion channel dynamics and electrophysiological properties. This study proposes the first artificial intelligence framework for characterizing multiple ion channel kinetics of whole‐cell recordings. The framework… read more here.
Keywords: channel kinetics; framework; patch clamp; ion ... See more keywords
The Mechanism FA-Dependent H+ Transport by UCP1.
Sign Up to like & getrecommendations! Published in 2019 at "Handbook of experimental pharmacology"
DOI: 10.1007/164_2018_138
Abstract: Uncoupling protein 1 (UCP1) is an integral protein of the inner mitochondrial membrane (IMM) that is expressed specifically in brown and beige fat depots. UCP1 is responsible for the production of heat to control core… read more here.
Keywords: mechanism; patch clamp; transport; mechanism dependent ... See more keywords
Nucleated, Outside-Out, Somatic, Macropatch Recordings in Native Neurons.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0818-0_11
Abstract: Patch-clamp recordings are a powerful tool for the live measurement of the plasma membrane biophysical properties, with the ability to discriminate fast events such as fast inactivating Na+ currents ( read more here.
Keywords: clamp recordings; patch clamp; native neurons; clamp ... See more keywords
Patch Clamp Technology in the Twenty-First Century.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0818-0_2
Abstract: In the almost four decades since its inception, the patch clamp technique has transitioned from a specialist skill to a method commonly used among many others in a lab. Development of patch clamp instrumentation has… read more here.
Keywords: clamp technology; technology twenty; twenty first; patch clamp ... See more keywords
Patch Clamp Analysis of Opioid-Induced Kir3 Currents in Mouse Peripheral Sensory Neurons Following Nerve Injury.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0884-5_12
Abstract: Patch clamp is an electrophysiological technique that allows to analyze the activity of ion channels in neurons. In this chapter, we provide a detailed description of patch clamp protocol to measure the effect of a μ-opioid… read more here.
Keywords: peripheral sensory; patch clamp; patch; sensory neurons ... See more keywords
Studying the Mechanism of Membrane Permeabilization Induced by Antimicrobial Peptides Using Patch-Clamp Techniques.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6737-7_18
Abstract: Many short peptides selectively permeabilize the bacteria plasma membrane, leading to their lyses and death: they are therefore a source of antibacterial molecules and inspiration for novel and more selective drugs, which may have wider… read more here.
Keywords: patch clamp; membrane; permeabilization; antimicrobial peptides ... See more keywords
Isolation and Patch-Clamp of Primary Adipocytes.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6820-6_14
Abstract: The patch-clamp technique allows for the study of ion channel activity in the native adipocyte environment to better understand the contributions of ion channels to adipocyte signaling. Here, we describe methods for isolating primary mature… read more here.
Keywords: clamp primary; patch clamp; patch; isolation patch ... See more keywords
Patch-Clamp Analysis of the Mitochondrial Calcium Uniporter.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9018-4_7
Abstract: Mitochondria accumulate significant amounts of calcium when cytosolic calcium is elevated above the resting levels of 50-100 nM during signaling events. This calcium uptake is primarily mediated by a macromolecular protein assembly called mitochondrial calcium uniporter… read more here.
Keywords: calcium; calcium uniporter; patch clamp; mitochondrial calcium ... See more keywords
Heterologous Expression and Patch-Clamp Recording of P2X Receptors in HEK293 Cells.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9717-6_19
Abstract: P2X receptors (P2XRs) are ligand-gated ion channels gated by extracellular adenosine 5'-triphosphate (ATP) and play a critical role in mediating ATP-induced purinergic signaling in physiological and pathological processes. Heterologous expression of P2XR in human embryonic… read more here.
Keywords: p2x receptors; patch clamp; hek293 cells; clamp recording ... See more keywords
Efficient, Automatic, and Reproducible Patch Clamp Data Analysis with “Auto ANT”, a User-Friendly Interface for Batch Analysis of Patch Clamp Recordings
Sign Up to like & getrecommendations! Published in 2025 at "Neuroinformatics"
DOI: 10.1007/s12021-025-09721-w
Abstract: Patch-clamp recordings are vital for investigating the electrical properties of excitable cells, yet the analysis of these recordings often involves time-consuming manual procedures prone to variability. To address this challenge, we developed the Auto ANT… read more here.
Keywords: auto ant; analysis; patch clamp;
Artifactual hyperpolarization during extracellular electrical stimulation: Proposed mechanism of high-rate neuromodulation disproved
Sign Up to like & getrecommendations! Published in 2018 at "Brain Stimulation"
DOI: 10.1016/j.brs.2017.12.004
Abstract: BACKGROUND Kilohertz-frequency electric field stimulation (kEFS) applied to the spinal cord can reduce chronic pain without causing the buzzing sensation (paresthesia) associated with activation of dorsal column fibers. This suggests that high-rate spinal cord stimulation… read more here.
Keywords: hyperpolarization; stimulation; patch clamp; extracellular electrical ... See more keywords