Ratiometric PCR in a Portable Sample-to-Result Device for Broad-Based Pathogen Identification.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c01357
Abstract: Polymerase chain reaction (PCR)-based diagnostic testing is the gold standard method for pathogen identification (ID) with recent developments enabling automated PCR tests for point-of-care (POC) use. However, multiplexed identification of several pathogens in PCR assays… read more here.
Keywords: poc ratiopcr; pathogen identification; identification; pcr ... See more keywords
Pathogen Identification: Ultrasensitive Nucleic Acid Detection via a Dynamic DNA Nanosystem-Integrated Ratiometric Electrochemical Sensing Strategy.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c04736
Abstract: Sensitively determining trace nucleic acid is of great significance for pathogen identification. Herein, a dynamic DNA nanosystem-integrated ratiometric electrochemical biosensor was proposed to determine human immunodeficiency virus-associated DNA fragment (HIV-DNA) with high sensitivity and selectivity.… read more here.
Keywords: dna nanosystem; dna; pathogen identification; nucleic acid ... See more keywords
A streamlined clinical metagenomic sequencing protocol for rapid pathogen identification.
Sign Up to like & getrecommendations! Published in 2021 at "Scientific reports"
DOI: 10.1038/s41598-021-83812-x
Abstract: Metagenomic next-generation sequencing (mNGS) holds promise as a diagnostic tool for unbiased pathogen identification and precision medicine. However, its medical utility depends largely on assay simplicity and reproducibility. In the current study, we aimed to… read more here.
Keywords: identification; metagenomic sequencing; pathogen identification; protocol rapid ... See more keywords
Pathogen identification by shotgun metagenomics of patients with necrotizing soft‐tissue infections
Sign Up to like & getrecommendations! Published in 2019 at "British Journal of Dermatology"
DOI: 10.1111/bjd.18611
Abstract: Necrotizing soft‐tissue infections (NSTIs) are life threatening, requiring broad‐spectrum antibiotics. Their aetiological diagnosis can be limited by poor performance of cultures and administration of antibiotics before surgery. read more here.
Keywords: necrotizing soft; identification shotgun; soft tissue; pathogen identification ... See more keywords
Advantages and Limitations of 16S rRNA Next-Generation Sequencing for Pathogen Identification in the Diagnostic Microbiology Laboratory: Perspectives from a Middle-Income Country
Sign Up to like & getrecommendations! Published in 2020 at "Diagnostics"
DOI: 10.3390/diagnostics10100816
Abstract: Bacterial culture and biochemical testing (CBtest) have been the cornerstone of pathogen identification in the diagnostic microbiology laboratory. With the advent of Sanger sequencing and later, next-generation sequencing, 16S rRNA next-generation sequencing (16SNGS) has been… read more here.
Keywords: middle income; diagnostic microbiology; microbiology; pathogen identification ... See more keywords