Rational approach to improve ansamitocin P‐3 production by integrating pathway engineering and substrate feeding in Actinosynnema pretiosum
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26775
Abstract: Ansamitocin P‐3 (AP‐3) produced by Actinosynnema pretiosum is an important antitumor agent for cancer treatment, but its market supply suffers from a low production titer. The role of AP‐3 unusual glycolate unit supply on its… read more here.
Keywords: production; pathway engineering; actinosynnema pretiosum; substrate feeding ... See more keywords
Protein and pathway engineering for the biosynthesis of 5‐hydroxytryptophan in Escherichia coli
Sign Up to like & getrecommendations! Published in 2017 at "Engineering in Life Sciences"
DOI: 10.1002/elsc.201700064
Abstract: The hydroxylation of tryptophan is an important reaction in the biosynthesis of natural products. 5‐Hydroxytryptophan (5HTP) is not only an important compound for its pharmaceutical value but also because it is the precursor of other… read more here.
Keywords: engineering; biosynthesis; protein pathway; biosynthesis hydroxytryptophan ... See more keywords
Transplanting the pathway engineering toolbox to methanogens.
Sign Up to like & getrecommendations! Published in 2019 at "Current opinion in biotechnology"
DOI: 10.1016/j.copbio.2019.02.009
Abstract: Biological methanogenesis evolved early in Earth's history and was likely already a major process by 3.5 Ga. Modern methanogenesis is now a key process in virtually all anaerobic microbial communities, such as marine and lake sediments,… read more here.
Keywords: toolbox; toolbox methanogens; pathway engineering; transplanting pathway ... See more keywords
Multimodular Pathway Engineering of Bacillus amyloliquefaciens for Intensifying Iturin A Production.
Sign Up to like & getrecommendations! Published in 2025 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.5c06329
Abstract: Iturin A is a potent lipopeptide with broad application potential. However, its microbial production remains constrained by limited precursor availability and metabolic inefficiencies. In this work, a modular metabolic engineering strategy was employed in Bacillus… read more here.
Keywords: engineering; multimodular pathway; bacillus amyloliquefaciens; pathway engineering ... See more keywords
Synergistic Improvement of 5-Aminolevulinic Acid Production with Synthetic Scaffolds and System Pathway Engineering.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00157
Abstract: Engineered synthetic scaffolds to organize metabolic pathway enzymes and system pathway engineering to fine-tune metabolic fluxes play essential roles in microbial production. Here, we first obtained the most favorable combination of key enzymes for 5-aminolevulinic… read more here.
Keywords: system; system pathway; production; synthetic scaffolds ... See more keywords
Pathway engineering in yeast for synthesizing the complex polyketide bikaverin
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-19984-3
Abstract: Fungal polyketides display remarkable structural diversity and bioactivity, and therefore the biosynthesis and engineering of this large class of molecules is therapeutically significant. Here, we successfully recode, construct and characterize the biosynthetic pathway of bikaverin,… read more here.
Keywords: engineering; bikaverin; pathway engineering; engineering yeast ... See more keywords
Pathway-engineering for highly-aligned block copolymer arrays.
Sign Up to like & getrecommendations! Published in 2017 at "Nanoscale"
DOI: 10.1039/c7nr06069f
Abstract: While the ultimate driving force in self-assembly is energy minimization and the corresponding evolution towards equilibrium, kinetic effects can also play a very strong role. These kinetic effects, such as trapping in metastable states, slow… read more here.
Keywords: block copolymer; highly aligned; pathway engineering; engineering highly ... See more keywords
Underground metabolism as a rich reservoir for pathway engineering.
Sign Up to like & getrecommendations! Published in 2022 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btac282
Abstract: MOTIVATION Bioproduction of value-added compounds is frequently achieved by utilizing enzymes from other species. However, expression of such heterologous enzymes can be detrimental due to unexpected interactions within the host cell. Recently, an alternative strategy… read more here.
Keywords: reservoir pathway; engineering; pathway engineering; underground metabolism ... See more keywords
Advanced pathway engineering for phototrophic putrescine production
Sign Up to like & getrecommendations! Published in 2022 at "Plant Biotechnology Journal"
DOI: 10.1111/pbi.13879
Abstract: Summary The polyamine putrescine (1,4‐diaminobutane) contributes to cellular fitness in most organisms, where it is derived from the amino acids ornithine or arginine. In the chemical industry, putrescine serves as a versatile building block for… read more here.
Keywords: advanced pathway; engineering phototrophic; production; pathway engineering ... See more keywords
Combinatorial biosynthesis of novel aminoglycoside antibiotics via pathway engineering
Sign Up to like & getrecommendations! Published in 2024 at "AMB Express"
DOI: 10.1186/s13568-024-01753-w
Abstract: With the current spread of clinically relevant multidrug-resistant (MDR) pathogens, insufficient unearthing of new anti-infectives, and the high cost required for approval of new antimicrobial agents, a strong need for getting these agents via more… read more here.
Keywords: pathway engineering; combinatorial biosynthesis; gene; aminoglycoside antibiotics ... See more keywords