Efficient isolation of protoplasts from rice calli with pause points and its application in transient gene expression and genome editing assays
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DOI: 10.1186/s13007-020-00692-4
Abstract: Background An efficient in vivo transient transfection system using protoplasts is an important tool to study gene expression, metabolic pathways, and multiple mutagenesis parameters in plants. Although rice protoplasts can be isolated from germinated seedlings… read more here.
Keywords: transfection; pause points; protoplast isolation; gene expression ... See more keywords