Developing a Cas9‐based tool to engineer native plasmids in Synechocystis sp. PCC 6803
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26747
Abstract: The oxygenic photosynthetic bacterium Synechocystis sp. PCC 6803 (S6803) is a model cyanobacterium widely used for fundamental research and biotechnology applications. Due to its polyploidy, existing methods for genome engineering of S6803 require multiple rounds… read more here.
Keywords: synechocystis pcc; pcc 6803; tool; native plasmids ... See more keywords
Effects of inactivation of the cyAbrB2 transcription factor together with glycogen synthesis on cellular metabolism and free fatty acid production in the cyanobacterium Synechocystis sp. PCC 6803
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26842
Abstract: Deletion of the cyAbrB2 (Sll0822) transcription factor in Synechocystis sp. PCC 6803 causes aberrant accumulation of glycogen. We previously tried to redirect the excess carbon stored as glycogen in the cyabrB2‐disrupted (∆ cyabrB2) mutant by… read more here.
Keywords: cyabrb2; pcc 6803; synechocystis pcc; transcription factor ... See more keywords
Effect of light fluctuations on photosynthesis and metabolic flux in Synechocystis sp. PCC 6803.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology progress"
DOI: 10.1002/btpr.3326
Abstract: In nature, photosynthetic organisms are exposed to fluctuating light, and their physiological systems must adapt to this fluctuation. To maintain homeostasis, these organisms have a light fluctuation photoprotective mechanism, which functions in both photosystems and… read more here.
Keywords: pcc 6803; synechocystis pcc; light fluctuations; metabolic flux ... See more keywords
Phosphate depletion controls lipid content and accumulation of heterotrophic bacteria during growth of Synechocystis sp. PCC 6803
Sign Up to like & getrecommendations! Published in 2019 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-019-09817-9
Abstract: During the culturing of cyanobacteria, heterotrophic bacteria can compete for nutrients, compromise the quality of the harvested biomass, or cause culture crashes. We systematically investigated the effects of depleting inorganic phosphate (Pi) on the growth… read more here.
Keywords: heterotrophic bacteria; pcc 6803; synechocystis pcc; growth ... See more keywords
Transcriptome and metabolome analyses of response of Synechocystis sp. PCC 6803 to methyl viologen.
Sign Up to like & getrecommendations! Published in 2021 at "Applied microbiology and biotechnology"
DOI: 10.1007/s00253-021-11628-w
Abstract: The toxicity of methyl viologen (MV) to organisms is mainly due to the oxidative stress caused by reactive oxygen species produced from cell response. This study mainly investigated the response of Synechocystis sp. PCC 6803… read more here.
Keywords: response; response synechocystis; pcc 6803; synechocystis pcc ... See more keywords
TonB-Dependent Utilization of Dihydroxamate Xenosiderophores in Synechocystis sp. PCC 6803
Sign Up to like & getrecommendations! Published in 2017 at "Current Microbiology"
DOI: 10.1007/s00284-017-1355-2
Abstract: In Gram-negative bacteria, transport of ferric siderophores through outer membrane is a complex process that requires specific outer membrane transporters and energy-transducing TonB–ExbB–ExbD system in the cytoplasmic membrane. The genome of the non-siderophore-producing cyanobacterium Synechocystis… read more here.
Keywords: tonb dependent; pcc 6803; synechocystis pcc; iron ... See more keywords
Characterization of ABC transporter genes, sll1180, sll1181, and slr1270, involved in acid stress tolerance of Synechocystis sp. PCC 6803
Sign Up to like & getrecommendations! Published in 2018 at "Photosynthesis Research"
DOI: 10.1007/s11120-018-0548-2
Abstract: Over 50 ATP-binding cassette (ABC) transporter-related genes are detected in the Synechocystis sp. PCC 6803 genome by genome sequence analysis. Deletion mutants of other substrate-unknown ABC transporter genes were screened for their acid stress sensitivities… read more here.
Keywords: synechocystis pcc; pcc 6803; stress; acid stress ... See more keywords
Effect of organic solvents on asymmetric reduction of β-keto esters using cyanobacterium Synechocystis sp. PCC 6803
Sign Up to like & getrecommendations! Published in 2021 at "Tetrahedron Letters"
DOI: 10.1016/j.tetlet.2021.153249
Abstract: Abstract The asymmetric reduction of tert-butyl 3-oxobutanoate by cyanobacterium Synechocystis sp. PCC 6803 under illumination with red LED light at 25 °C for 24 h afforded the corresponding (R)-β-hydroxy ester in 79% enantiomeric excess (ee) (81% yield),… read more here.
Keywords: organic solvents; pcc 6803; cyanobacterium synechocystis; synechocystis pcc ... See more keywords
Light-Regulated Natural Fluorescence of the PCC 6803@ZIF-8 Composite as an Encoded Microsphere for the Detection of Multiple Biomarkers.
Sign Up to like & getrecommendations! Published in 2021 at "ACS sensors"
DOI: 10.1021/acssensors.1c00104
Abstract: The use of color-encoded microspheres for a bead-based assay has attracted increasing attention for high-throughput multiplexed bioassays. A fluorescent PCC 6803@ZIF-8 composite was prepared as a bead-based assay platform by a self-assembled zeolitic imidazolate framework… read more here.
Keywords: fluorescence; pcc 6803; zif composite; 6803 zif ... See more keywords
Construction of Fully Segregated Genomic Libraries in Polyploid Organisms Such as Synechocystis sp. PCC 6803
Sign Up to like & getrecommendations! Published in 2020 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.0c00353
Abstract: Several microbes are polyploid, meaning they contain several copies of their chromosome. Cyanobacteria, while holding great potential as photosynthetic cell factories of various products, are found among them. In these clades the diversity of genetic… read more here.
Keywords: genomic libraries; pcc 6803; fully segregated; synechocystis pcc ... See more keywords
Interlaboratory Reproducibility in Growth and Reporter Expression in the Cyanobacterium Synechocystis sp. PCC 6803.
Sign Up to like & getrecommendations! Published in 2023 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.3c00150
Abstract: In recent years, a plethora of new synthetic biology tools for use in cyanobacteria have been published; however, their reported characterizations often cannot be reproduced, greatly limiting the comparability of results and hindering their applicability.… read more here.
Keywords: pcc 6803; reproducibility; growth; synechocystis pcc ... See more keywords