Pathologic Complete Response and Clinical Outcomes in Patients With Localized Soft Tissue Sarcoma Treated With Neoadjuvant Chemoradiotherapy or Radiotherapy: The NRG/RTOG 9514 and 0630 Nonrandomized Clinical Trials.
Sign Up to like & getrecommendations! Published in 2023 at "JAMA oncology"
DOI: 10.1001/jamaoncol.2023.0042
Abstract: Importance Pathologic complete response (pCR) may be associated with prognosis in patients with soft tissue sarcoma (STS). Objective We sought to determine the prognostic significance of pCR on survival outcomes in STS for patients receiving… read more here.
Keywords: rtog 0630; oncology; clinical trials; rtog ... See more keywords
Assessment of the HER2DX Assay in Patients With ERBB2-Positive Breast Cancer Treated With Neoadjuvant Paclitaxel, Trastuzumab, and Pertuzumab.
Sign Up to like & getrecommendations! Published in 2023 at "JAMA oncology"
DOI: 10.1001/jamaoncol.2023.0181
Abstract: Importance Patients with early-stage ERBB2 (formerly HER2)-positive breast cancer (ERBB2+ BC) who experience a pathologic complete response (pCR) after receiving neoadjuvant therapy have favorable survival outcomes. Predicting the likelihood of pCR may help optimize neoadjuvant… read more here.
Keywords: her2dx assay; her2dx pcr; pcr score; pcr ... See more keywords
Assessment of a Genomic Assay in Patients With ERBB2-Positive Breast Cancer Following Neoadjuvant Trastuzumab-Based Chemotherapy With or Without Pertuzumab.
Sign Up to like & getrecommendations! Published in 2023 at "JAMA oncology"
DOI: 10.1001/jamaoncol.2023.0187
Abstract: Importance Biomarkers to guide the use of pertuzumab in the treatment of early-stage ERBB2 (formerly HER2)-positive breast cancer beyond simple ERBB2 status are needed. Objective To determine if use of the HER2DX genomic assay (Reveal… read more here.
Keywords: assay; assay reported; breast cancer; pcr ... See more keywords
Evaluation of two new highly multiplexed PCR assays as an alternative to next‐generation sequencing for IDH1/2 mutation detection
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Oncology"
DOI: 10.1002/1878-0261.13311
Abstract: IDH1 and IDH2 somatic mutations have been identified in solid tumors and blood malignancies. The development of inhibitors of mutant IDH1 and IDH2 in the past few years has prompted the development of a fast… read more here.
Keywords: multiplexed pcr; two new; detection; next generation ... See more keywords
Multiplex Digital Methylation‐Specific PCR for Noninvasive Screening of Lung Cancer
Sign Up to like & getrecommendations! Published in 2023 at "Advanced Science"
DOI: 10.1002/advs.202206518
Abstract: There remains tremendous interest in developing liquid biopsy assays for detection of cancer‐specific alterations, such as mutations and DNA methylation, in cell‐free DNA (cfDNA) obtained through noninvasive blood draws. However, liquid biopsy analysis is often… read more here.
Keywords: methylation; multiplex digital; digital methylation; pcr ... See more keywords
Ultrasensitive NGS MRD assessment in Ph+ ALL: Prognostic impact and correlation with RT-PCR for BCR::ABL1.
Sign Up to like & getrecommendations! Published in 2023 at "American journal of hematology"
DOI: 10.1002/ajh.26949
Abstract: Reverse transcription polymerase chain reaction (RT-PCR) for BCR::ABL1 is the most common and widely accepted method of measurable residual disease (MRD) assessment in Philadelphia chromosome-positive acute lymphoblastic leukemia (Ph+ ALL); however, RT-PCR may not be an… read more here.
Keywords: ngs mrd; pcr bcr; pcr; bcr abl1 ... See more keywords
Using acetone for rapid PCR‐amplifiable DNA extraction from recalcitrant woody plant taxa
Sign Up to like & getrecommendations! Published in 2020 at "Applications in Plant Sciences"
DOI: 10.1002/aps3.11403
Abstract: Premise Quick and effective DNA extraction from plants for subsequent PCR amplification is sometimes challenging when working across diverse plant taxa that may contain a variety of inhibitory compounds. Time‐consuming methods may be needed to… read more here.
Keywords: extraction; dna; pcr; dna extraction ... See more keywords
Integrating tissue‐direct PCR into genetic identification: An upgraded molecular ecology approach to survey fern gametophytes in the field
Sign Up to like & getrecommendations! Published in 2022 at "Applications in Plant Sciences"
DOI: 10.1002/aps3.11462
Abstract: Abstract Premise The gametophytes of different fern species collected in the field can be difficult to distinguish because of their morphological similarities. Nonetheless, emerging molecular ecology techniques are starting to be used to tackle such… read more here.
Keywords: ecology; identification; pcr; field ... See more keywords
Highly sensitive detection of driver mutations from cytological samples and cfDNA in lung cancer.
Sign Up to like & getrecommendations! Published in 2021 at "Cancer medicine"
DOI: 10.1002/cam4.4330
Abstract: BACKGROUND Bronchoscopy is a minimally invasive procedure for establishing the diagnosis of lung cancer. It sometimes fails to obtain tissue samples but readily collects cytological samples. METHODS We developed PNA-LNA dual-PCR (PLDP), which amplified mutant… read more here.
Keywords: cytological samples; pldp; pcr; lung cancer ... See more keywords
Thermally resistant nuclease in Serratia marcescens hinders PCR reactions and degrades PCR products
Sign Up to like & getrecommendations! Published in 2024 at "Cell Biochemistry and Function"
DOI: 10.1002/cbf.4032
Abstract: Polymerase chain reaction (PCR) is an important tool for exogenous gene acquisition and recombinants identification. There exist two problems when using Serratia marcescens as a template for PCR amplification: amplified PCR products are rapidly degraded,… read more here.
Keywords: pcr products; degrades pcr; pcr; thermally resistant ... See more keywords
Comparative Analysis of Two Digital PCR Platforms for Detecting DNA Methylation in Patient Samples
Sign Up to like & getrecommendations! Published in 2025 at "Cell Biochemistry and Function"
DOI: 10.1002/cbf.70112
Abstract: The choice of appropriate analytical methods for determining methylation patterns at specific loci across the genome is essential for identifying novel diagnostic and prognostic markers for subsequent clinical implementation. Various methods exist for determining methylation… read more here.
Keywords: methylation; dna methylation; analysis; pcr ... See more keywords