Trehalose improves PCR amplification of vertebrate nuclear DNA from historical allozymes
Sign Up to like & getrecommendations! Published in 2017 at "Conservation Genetics Resources"
DOI: 10.1007/s12686-017-0811-4
Abstract: Historical tissue collections represent potential resources for temporal genetic studies in evolutionary and conservation biology. Unfortunately, DNA from historical samples stored without modern genetic analyses in mind, such as frozen allozyme homogenates, are often degraded… read more here.
Keywords: pcr amplification; vertebrate nuclear; nuclear dna; trehalose improves ... See more keywords
A practical study on direct PCR amplification using the GlobalFiler™ PCR Amplification Kit on human bloodstains collected with microFLOQ™ Direct swabs.
Sign Up to like & getrecommendations! Published in 2019 at "Forensic science international"
DOI: 10.1016/j.forsciint.2019.04.018
Abstract: Rapid DNA profiling of casework samples is a powerful tool that can support law enforcement agencies in the quick apprehension of perpetrators before they re-offend or escape the jurisdiction. This present study evaluated the feasibility… read more here.
Keywords: pcr amplification; direct pcr; dna; microfloq direct ... See more keywords
Performance evaluation of fungal DNA PCR amplification from formalin‐fixed paraffin‐embedded tissue for diagnosis: Experience of a tertiary reference laboratory
Sign Up to like & getrecommendations! Published in 2021 at "Mycoses"
DOI: 10.1111/myc.13249
Abstract: Diagnosis of invasive fungal infections from formalin‐fixed paraffin‐embedded (FFPE) tissues by PCR amplification is a developing technology. One of the difficulties of establishing a validated protocol for this testing is that the gold standard, culture,… read more here.
Keywords: fixed paraffin; pcr amplification; diagnosis; paraffin embedded ... See more keywords
DMSO and betaine significantly enhance the PCR amplification of ITS2 DNA barcodes from plants.
Sign Up to like & getrecommendations! Published in 2020 at "Genome"
DOI: 10.1139/gen-2019-0221
Abstract: ITS2 marker is highly efficient in species discrimination but its application in DNA barcoding is limited due to huge variations in the PCR success rate. We have hypothesized that higher GC content and the resultant… read more here.
Keywords: success rate; betaine; pcr success; pcr amplification ... See more keywords
Assessing alternative base substitutions at primer CpG sites to optimise unbiased PCR amplification of methylated sequences
Sign Up to like & getrecommendations! Published in 2017 at "Clinical Epigenetics"
DOI: 10.1186/s13148-017-0328-4
Abstract: BackgroundDetermining the role of DNA methylation in various biological processes is dependent on the accurate representation of often highly complex patterns. Accurate representation is dependent on unbiased PCR amplification post bisulfite modification, regardless of methylation status… read more here.
Keywords: methylation; pcr amplification; amplification methylated; pcr ... See more keywords
PCR amplification of a long rDNA segment with one primer pair in agriculturally important nematodes
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Nematology"
DOI: 10.21307/jofnem-2019-026
Abstract: Abstract Ribosomal DNA has been a reliable source of taxonomic and phylogenetic markers due to its high copy number in the genome and stable variation with few polymorphisms due to the homogenizing effect of concerted… read more here.
Keywords: pcr amplification; primer; segment; 28s 28s ... See more keywords