Simultaneous detection of Zika, chikungunya, dengue, yellow fever, West Nile, and Japanese encephalitis viruses by a two‐tube multiplex real‐time RT‐PCR assay
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Medical Virology"
DOI: 10.1002/jmv.27658
Abstract: Due to the concurrent prevalence and increasing risk of coinfection of the clinically important Arboviruses, timely and accurate differential diagnosis is important for clinical management and the epidemiological investigation. A two‐tube multiplex real‐time reverse transcription‐polymerase… read more here.
Keywords: tube multiplex; detection; pcr assay; two tube ... See more keywords
Evaluation and validation of an RT‐PCR assay for specific detection of monkeypox virus (MPXV)
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Medical Virology"
DOI: 10.1002/jmv.28247
Abstract: Monkeypox virus (MPXV) is a zoonotic orthopoxvirus within the Poxviridae family. MPXV is endemic to Central and West Africa. However, the world is currently witnessing an international outbreak with no clear epidemiological links to travel… read more here.
Keywords: virus mpxv; pcr assay; monkeypox virus;
Identification of spermatozoa using a novel 3-plex MSRE-PCR assay for forensic examination of sexual assaults
Sign Up to like & getrecommendations! Published in 2020 at "International Journal of Legal Medicine"
DOI: 10.1007/s00414-020-02285-1
Abstract: Identification of semen and then spermatozoa is essential to verify that sexual activity has occurred in alleged cases of sexual assault. Microscopic examination commonly used for spermatozoa identification is however time-consuming and can often lead… read more here.
Keywords: spermatozoa; pcr assay; plex msre; msre pcr ... See more keywords
Development of a TaqMan MGB RT-PCR assay for the detection of type A and subtype H10 avian influenza viruses
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DOI: 10.1007/s00705-018-3889-4
Abstract: H10 subtype avian influenza viruses have caused several epidemics in poultry and mammals, and specific, rapid and sensitive methods for detection are urgently needed. Herein, TaqMan minor groove binder (MGB) probes and multiplex real-time RT-PCR… read more here.
Keywords: avian influenza; detection; h10; influenza viruses ... See more keywords
Development of a Species-Specific PCR Assay for Correct Labeling of Eight Squid Species from Loliginidae and Ommastrephidae Families (Mollusca: Cephalopoda)
Sign Up to like & getrecommendations! Published in 2018 at "Food Analytical Methods"
DOI: 10.1007/s12161-018-1287-x
Abstract: Squid is an important seafood resource in Asian and European countries. Due to the different nutritional and market values of various squid species, substitutions between species often occur for higher profits. In this study, a… read more here.
Keywords: specific pcr; eight squid; pcr assay; squid species ... See more keywords
Development of multiplex PCR assay for simultaneous detection of five cucumber pathogens based on comparative genomics
Sign Up to like & getrecommendations! Published in 2019 at "Australasian Plant Pathology"
DOI: 10.1007/s13313-019-00637-z
Abstract: The aim of this study was to develop a multiplex PCR assay for rapid, reliable and simultaneous detection of five important cucumber pathogens: Fusarium oxysporum, F. solani, Sclerotinia sclerotiorum, Pythium aphanidermatum and Botrytis cinerea. One… read more here.
Keywords: pcr assay; simultaneous detection; cucumber pathogens; multiplex pcr ... See more keywords
Development and validation of multiplex-PCR assay to simultaneously detect favourable alleles of shrunken2, opaque2, crtRB1 and lcyE genes in marker-assisted selection for maize biofortification
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Plant Biochemistry and Biotechnology"
DOI: 10.1007/s13562-020-00585-6
Abstract: Sweet corn has emerged as a popular vegetable worldwide. Commercial shrunken2 (sh2)-based sweet corn lacks lysine, tryptophan and provitamin-A, deficiency of which cause various health problems. To overcome these problems, marker-assisted stacking of sh2, opaque2… read more here.
Keywords: marker assisted; crtrb1 lcye; crtrb1; pcr assay ... See more keywords
A seminested PCR assay for detection and typing of human papillomavirus based on E1 gene sequences.
Sign Up to like & getrecommendations! Published in 2018 at "Diagnostic microbiology and infectious disease"
DOI: 10.1016/j.diagmicrobio.2017.12.016
Abstract: HPV infection is considered one of the leading causes of cervical cancer in the world. To date, more than 180 types of HPV have been described and viral typing is critical for defining the prognosis… read more here.
Keywords: assay detection; detection typing; pcr assay; seminested pcr ... See more keywords
Direct pentaplex PCR assay: An adjunct panel for meat species identification in Asian food products.
Sign Up to like & getrecommendations! Published in 2019 at "Food chemistry"
DOI: 10.1016/j.foodchem.2018.07.143
Abstract: A direct pentaplex PCR assay was developed for the identification of meat from sources other than those declared on the packaging. Species-specific primers were designed, based on the mitochondrial cytochrome oxidase I (COI) gene. The… read more here.
Keywords: direct pentaplex; food products; pcr assay; meat ... See more keywords
High-resolution melt curve PCR assay for specific detection of E. coli O157:H7 in beef
Sign Up to like & getrecommendations! Published in 2018 at "Food Control"
DOI: 10.1016/j.foodcont.2017.11.025
Abstract: Abstract Among the disease-causing Shiga toxin-producing Escherichia coli (STEC), E. coli O157:H7 is estimated to cause one-third of the total STEC illnesses and the most cases of hemolytic uremic syndrome (HUS) in the U.S. The uidA… read more here.
Keywords: beef; high resolution; coli o157; pcr assay ... See more keywords
SYBR-Green real-time PCR assay with melting curve analysis for the rapid identification of Mytilus species in food samples
Sign Up to like & getrecommendations! Published in 2021 at "Food Control"
DOI: 10.1016/j.foodcont.2021.108257
Abstract: Abstract Mytilus spp. mussels are one of the most commonly cultivated species in the European Union, being M. galloprovincialis, M. edulis and M. chilensis the main species marketed here. Therefore, the identification of this seafood… read more here.
Keywords: methodology; identification mytilus; real time; pcr assay ... See more keywords