Evaluation of two new highly multiplexed PCR assays as an alternative to next‐generation sequencing for IDH1/2 mutation detection
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Oncology"
DOI: 10.1002/1878-0261.13311
Abstract: IDH1 and IDH2 somatic mutations have been identified in solid tumors and blood malignancies. The development of inhibitors of mutant IDH1 and IDH2 in the past few years has prompted the development of a fast… read more here.
Keywords: multiplexed pcr; two new; detection; next generation ... See more keywords
Comparison of three multiplex PCR assays for detection of respiratory viruses: Anyplex II RV16, AdvanSure RV, and Real‐Q RV
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Clinical Laboratory Analysis"
DOI: 10.1002/jcla.22230
Abstract: Due to its great sensitivity, the nucleic acid amplification test (NAAT) is widely used for detection of respiratory viruses (RV). However, few reports have described a direct comparison between multiplex RT‐PCR assays for RV. The… read more here.
Keywords: detection respiratory; respiratory viruses; pcr assays; multiplex pcr ... See more keywords
Evaluation of the performance of two real‐time PCR assays for detecting Mycobacterium species
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Clinical Laboratory Analysis"
DOI: 10.1002/jcla.22645
Abstract: Rapid discrimination between Mycobacterium tuberculosis (MTB) and nontuberculous mycobacteria (NTM) is critical for patient treatment and to avoid unnecessary expenditure on infection control. Because real‐time PCR assays distinguish MTB from NTM, we evaluated the performance… read more here.
Keywords: time pcr; pcr assays; performance two; real time ... See more keywords
Evaluation of real-time RT-PCR assays for detection and quantification of norovirus genogroups I and II
Sign Up to like & getrecommendations! Published in 2017 at "Virologica Sinica"
DOI: 10.1007/s12250-016-3863-9
Abstract: Noroviruses are the leading cause of acute gastroenteritis in humans. Real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) is a promising molecular method for the detection of noroviruses. In this study, the performance of three TaqMan… read more here.
Keywords: real time; gii; time pcr; time ... See more keywords
Applications of gold nanoparticles in ELISA, PCR, and immuno-PCR assays: A review.
Sign Up to like & getrecommendations! Published in 2021 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2020.08.030
Abstract: Development of state-of-the-art assays for sensitive and specific detection of disease biomarkers has received significant interest for early detection and prevention of various diseases. Enzyme Linked Immunosorbent assays (ELISA) and Polymerase Chain Reaction (PCR) are… read more here.
Keywords: pcr assays; detection; gold nanoparticles; immuno pcr ... See more keywords
Real-time PCR assays for the quantitative detection of Kazachstania servazzii and Candida sake related to undesirable white colony on kimchi
Sign Up to like & getrecommendations! Published in 2021 at "Food Control"
DOI: 10.1016/j.foodcont.2021.107984
Abstract: Abstract Kazachstania servazzii and Candia sake are involved in kimchi fermentation and form undesirable white colonies on the kimchi surface in the late stage of fermentation. To quantify the presence of these two yeast species,… read more here.
Keywords: real time; servazzii; pcr assays; time pcr ... See more keywords
Molecular detection of hemogregarines and haemosporidians in Brazilian free-living testudines
Sign Up to like & getrecommendations! Published in 2018 at "International Journal for Parasitology: Parasites and Wildlife"
DOI: 10.1016/j.ijppaw.2018.01.008
Abstract: Morphological and molecular techniques were used to investigate the presence of hemogregarines and haemosporidians in biological samples of free-living Geoffroy's side-necked turtles (Phrynops geoffroanus) and Giant Amazon turtles (Podocnemis expansa) from Brazil. No evolutionary form… read more here.
Keywords: detection hemogregarines; molecular detection; hemogregarines haemosporidians; free living ... See more keywords
New colony multiplex PCR assays for the detection and discrimination of vancomycin-resistant enterococcal species.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of microbiological methods"
DOI: 10.1016/j.mimet.2017.12.013
Abstract: New colony multiplex PCR assays for detection of seven types of vancomycin-resistance determinants and eight types of Enterococcus species were developed. For 135 enterococcal isolates examined in this study, these assays showed high sensitivity and… read more here.
Keywords: detection; multiplex pcr; new colony; pcr assays ... See more keywords
Development and validation of real-time PCR assays for the detection of Ehrlichia species and E. chaffeensis in clinical specimens.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of microbiological methods"
DOI: 10.1016/j.mimet.2021.106225
Abstract: Ehrlichiosis, caused by Gram-negative bacteria of the genus Ehrlichia, is considered an emerging infectious disease due to the increasing number of reported cases. Symptoms are non-specific and occur within 1 to 2 weeks following the bite… read more here.
Keywords: ehrlichia; real time; time pcr; pcr ... See more keywords
Rapid screening for SARS-CoV-2 variants of concern in clinical and environmental samples using nested RT-PCR assays targeting key mutations of the spike protein
Sign Up to like & getrecommendations! Published in 2021 at "Water Research"
DOI: 10.1016/j.watres.2021.117104
Abstract: New SARS-CoV-2 mutations are constantly emerging, raising concerns of increased transmissibility, virulence or escape from host immune response. We describe a nested RT-PCR assay (∼1500 bps) to detect multiple nucleotide changes resulting in key spike… read more here.
Keywords: cov variants; spike protein; sars cov; pcr assays ... See more keywords
Validation of species-specific PCR assays for the detection of Pantoea ananatis, P. agglomerans, P. allii and P. stewartii.
Sign Up to like & getrecommendations! Published in 2022 at "Plant disease"
DOI: 10.1094/pdis-08-21-1810-sc
Abstract: Species of Pantoea represent a group of plant pathogenic bacteria that infect a variety of agro-economically important plant species. Among these, a complex of P. ananatis, P. allii, P. agglomerans, and P. stewartii subsp. indologenes… read more here.
Keywords: pantoea; agglomerans allii; ananatis; pcr assays ... See more keywords