Using a PCR‐Based Method To Analyze and Model Large, Heterogeneous Populations of DNA†
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DOI: 10.1002/cbic.201900603
Abstract: The study of populations of large size and high diversity is limited by the capability of collecting data. Moreover, for a pool of individuals, each associated with a unique characteristic feature, as the pool size… read more here.
Keywords: diversity; dna; pcr based; method ... See more keywords
Developing a multiplex PCR‐based assay kit for bloodstream infection by analyzing genomic big data
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Clinical Laboratory Analysis"
DOI: 10.1002/jcla.24686
Abstract: In recent years, the incidence of bloodstream infections (BSI) has increased, the composition of pathogenic bacteria has changed, and drug resistance among bacteria has gradually increased due to the widespread use of interventional techniques, broad‐spectrum… read more here.
Keywords: bloodstream; assay kit; developing multiplex; pcr based ... See more keywords
Detection of gastroenteritis viruses among pediatric patients in Hiroshima Prefecture, Japan, between 2006 and 2013 using multiplex reverse transcription PCR‐based assays involving fluorescent dye‐labeled primers
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Medical Virology"
DOI: 10.1002/jmv.24714
Abstract: Multiplex reverse transcription (RT)‐polymerase chain reaction (PCR)‐based assays involving fluorescent dye‐labeled primers were modified to detect 10 types of gastroenteritis viruses by adding two further assays to a previously developed assay. Then, these assays were… read more here.
Keywords: based assays; involving fluorescent; assays involving; fluorescent dye ... See more keywords
Head‐to‐head comparison of genotyping of human papillomavirus by GP5+/6+‐PCR‐based reverse dot blot hybridization assay and SPF10‐PCR‐based line probe assay
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Medical Virology"
DOI: 10.1002/jmv.28435
Abstract: The SPF10‐PCR‐based line probe assay (LiPA‐25) for human papillomavirus (HPV) genotyping with high analytical sensitivity and specificity was widely used in HPV vaccine clinical trials and epidemiologic studies. In the study, we aimed to compare… read more here.
Keywords: based line; head; hpv; pcr ... See more keywords
Head‐to‐head comparison of genotyping of human papillomavirus by real‐time multiplex PCR assay using type‐specific primers and SPF10‐PCR‐based line probe assay
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Medical Virology"
DOI: 10.1002/jmv.28579
Abstract: The SPF10‐polymerase chain reaction (PCR)‐based line probe assay (LiPA‐25) with high analytical sensitivity and specificity for human papillomavirus (HPV) genotyping in clinical samples has been widely used in vaccine and epidemiologic studies. A real‐time multiplex… read more here.
Keywords: agreement; based line; head; hybribio ... See more keywords
PCR-based diagnosis is not always useful in the acute acquired toxoplasmosis in immunocompetent individuals
Sign Up to like & getrecommendations! Published in 2021 at "Parasitology Research"
DOI: 10.1007/s00436-020-07022-6
Abstract: Toxoplasmosis is the most prevalent zoonosis in the world and is associated with a large spectrum of diseases. Acute acquired toxoplasmosis (AAT) is considered a benign and self-limiting disease but severe postnatal infections have been… read more here.
Keywords: diagnosis; acute acquired; toxoplasmosis; acquired toxoplasmosis ... See more keywords
A pilot study on PCR-based detection of four foodborne pathogenic microorganisms
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DOI: 10.1007/s11694-017-9680-5
Abstract: To establish PCR-based detection methods for Staphylococcus aureus, Shigella, Pasteurella multocida and Pseudomonas aeruginosa, the nuc, ipah, ptfa and oprl genes were amplified by singleplex PCRs and multiplex PCR using specific primers that were designed… read more here.
Keywords: detection; singleplex pcrs; pcr; based detection ... See more keywords
Educational no. 4: PCR-based methods
Sign Up to like & getrecommendations! Published in 2018 at "memo - Magazine of European Medical Oncology"
DOI: 10.1007/s12254-018-0460-7
Abstract: SummaryFor decades, polymerase chain reaction (PCR) has been playing a fundamental role in hematology. Not only in diagnostics, but also in follow-up and therapeutic decision-making, PCR impacts the treatment of patients with neoplastic diseases. In this… read more here.
Keywords: educational pcr; hematology; based methods; oncology ... See more keywords
PCR-based species identification applied in Japanese pear orchards to survey seasonal proportion changes of phytoseiid mite species
Sign Up to like & getrecommendations! Published in 2018 at "Applied Entomology and Zoology"
DOI: 10.1007/s13355-018-0595-3
Abstract: For this study, we developed a PCR-based method to identify the ribosomal internal transcribed spacer (ITS) sequences of six phytoseiid mite species found in Japanese pear orchards: Neoseiulus californicus (McGregor), Neoseiulus womersleyi (Schicha), Neoseiulus makuwa… read more here.
Keywords: pear orchards; mite species; japanese pear; phytoseiid mite ... See more keywords
A simple PCR-based approach for rapid detection of Ips typographus and Ips duplicatus in the presence of (associated) symbionts and parasites
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Plant Diseases and Protection"
DOI: 10.1007/s41348-020-00388-w
Abstract: In plant pest diagnosis, Sanger sequencing of marker genes (DNA-barcoding) is the most applied and appropriate method for the identification of insects. Standard PM7/129 of the European and Mediterranean Plant Protection Organization (EPPO) includes a… read more here.
Keywords: approach rapid; simple pcr; ips duplicatus; barcoding region ... See more keywords
PCR-based detection of Toxoplasma gondii from cattle in southern Iran.
Sign Up to like & getrecommendations! Published in 2021 at "Comparative immunology, microbiology and infectious diseases"
DOI: 10.1016/j.cimid.2021.101677
Abstract: OBJECTIVE Toxoplasma gondii is a protozoan parasite that is widely prevalent in most warm-blooded vertebrates. Humans mainly become infected by eating raw or undercooked meat. This study was designed to investigate the infection of cattle… read more here.
Keywords: pcr based; toxoplasma gondii; infection; southern iran ... See more keywords