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   Articles with "pcr based" as a keyword



Microsatellite instability evaluation by a novel PCR‐based 8‐loci test kit in colorectal cancer

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recommendations! Published in 2024 at "Biotechnology and Applied Biochemistry"

DOI: 10.1002/bab.2582

Abstract: Microsatellite instability (MSI) assessment is strongly recommended for colorectal cancer patients, as MSI status is crucial in determining optimal treatment and predicting prognosis. This study evaluated the reliability and accuracy of a novel polymerase chain… read more here.

Keywords: test kit; novel pcr; pcr based; based loci ... See more keywords
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Using a PCR‐Based Method To Analyze and Model Large, Heterogeneous Populations of DNA†

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recommendations! Published in 2020 at "Chembiochem"

DOI: 10.1002/cbic.201900603

Abstract: The study of populations of large size and high diversity is limited by the capability of collecting data. Moreover, for a pool of individuals, each associated with a unique characteristic feature, as the pool size… read more here.

Keywords: diversity; dna; pcr based; method ... See more keywords

Developing a multiplex PCR‐based assay kit for bloodstream infection by analyzing genomic big data

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recommendations! Published in 2022 at "Journal of Clinical Laboratory Analysis"

DOI: 10.1002/jcla.24686

Abstract: In recent years, the incidence of bloodstream infections (BSI) has increased, the composition of pathogenic bacteria has changed, and drug resistance among bacteria has gradually increased due to the widespread use of interventional techniques, broad‐spectrum… read more here.

Keywords: bloodstream; assay kit; developing multiplex; pcr based ... See more keywords
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Detection of gastroenteritis viruses among pediatric patients in Hiroshima Prefecture, Japan, between 2006 and 2013 using multiplex reverse transcription PCR‐based assays involving fluorescent dye‐labeled primers

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recommendations! Published in 2017 at "Journal of Medical Virology"

DOI: 10.1002/jmv.24714

Abstract: Multiplex reverse transcription (RT)‐polymerase chain reaction (PCR)‐based assays involving fluorescent dye‐labeled primers were modified to detect 10 types of gastroenteritis viruses by adding two further assays to a previously developed assay. Then, these assays were… read more here.

Keywords: based assays; involving fluorescent; assays involving; fluorescent dye ... See more keywords

Head‐to‐head comparison of genotyping of human papillomavirus by GP5+/6+‐PCR‐based reverse dot blot hybridization assay and SPF10‐PCR‐based line probe assay

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recommendations! Published in 2022 at "Journal of Medical Virology"

DOI: 10.1002/jmv.28435

Abstract: The SPF10‐PCR‐based line probe assay (LiPA‐25) for human papillomavirus (HPV) genotyping with high analytical sensitivity and specificity was widely used in HPV vaccine clinical trials and epidemiologic studies. In the study, we aimed to compare… read more here.

Keywords: based line; head; hpv; pcr ... See more keywords

Head‐to‐head comparison of genotyping of human papillomavirus by real‐time multiplex PCR assay using type‐specific primers and SPF10‐PCR‐based line probe assay

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recommendations! Published in 2023 at "Journal of Medical Virology"

DOI: 10.1002/jmv.28579

Abstract: The SPF10‐polymerase chain reaction (PCR)‐based line probe assay (LiPA‐25) with high analytical sensitivity and specificity for human papillomavirus (HPV) genotyping in clinical samples has been widely used in vaccine and epidemiologic studies. A real‐time multiplex… read more here.

Keywords: agreement; based line; head; hybribio ... See more keywords

A simple and reliable PCR-based method to differentiate between XX and XY sex genotypes in Cannabis sativa

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recommendations! Published in 2025 at "Planta"

DOI: 10.1007/s00425-025-04804-z

Abstract: Cannabis sativa is mainly a dioecious plant, which means that female and male flowers develop on separate individuals, which is controlled by an XY sex determination system: females have two X chromosomes and male plants… read more here.

Keywords: sex; cannabis sativa; based method; pcr based ... See more keywords

PCR-based diagnosis is not always useful in the acute acquired toxoplasmosis in immunocompetent individuals

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recommendations! Published in 2021 at "Parasitology Research"

DOI: 10.1007/s00436-020-07022-6

Abstract: Toxoplasmosis is the most prevalent zoonosis in the world and is associated with a large spectrum of diseases. Acute acquired toxoplasmosis (AAT) is considered a benign and self-limiting disease but severe postnatal infections have been… read more here.

Keywords: diagnosis; acute acquired; toxoplasmosis; acquired toxoplasmosis ... See more keywords

A pilot study on PCR-based detection of four foodborne pathogenic microorganisms

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recommendations! Published in 2017 at "Journal of Food Measurement and Characterization"

DOI: 10.1007/s11694-017-9680-5

Abstract: To establish PCR-based detection methods for Staphylococcus aureus, Shigella, Pasteurella multocida and Pseudomonas aeruginosa, the nuc, ipah, ptfa and oprl genes were amplified by singleplex PCRs and multiplex PCR using specific primers that were designed… read more here.

Keywords: detection; singleplex pcrs; pcr; based detection ... See more keywords
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Educational no. 4: PCR-based methods

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recommendations! Published in 2018 at "memo - Magazine of European Medical Oncology"

DOI: 10.1007/s12254-018-0460-7

Abstract: SummaryFor decades, polymerase chain reaction (PCR) has been playing a fundamental role in hematology. Not only in diagnostics, but also in follow-up and therapeutic decision-making, PCR impacts the treatment of patients with neoplastic diseases. In this… read more here.

Keywords: educational pcr; hematology; based methods; oncology ... See more keywords

PCR-based species identification applied in Japanese pear orchards to survey seasonal proportion changes of phytoseiid mite species

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recommendations! Published in 2018 at "Applied Entomology and Zoology"

DOI: 10.1007/s13355-018-0595-3

Abstract: For this study, we developed a PCR-based method to identify the ribosomal internal transcribed spacer (ITS) sequences of six phytoseiid mite species found in Japanese pear orchards: Neoseiulus californicus (McGregor), Neoseiulus womersleyi (Schicha), Neoseiulus makuwa… read more here.

Keywords: pear orchards; mite species; japanese pear; phytoseiid mite ... See more keywords