PCR detection for syphilis diagnosis: Status and prospects
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Clinical Laboratory Analysis"
DOI: 10.1002/jcla.22890
Abstract: Syphilis, a re‐emerging public health problem worldwide caused by Treponema pallidum subsp pallidum (T. pallidum), usually induces systemic and chronic inflammation in hosts who do not receive timely therapy after exposing to high‐risk factors such… read more here.
Keywords: diagnosis status; detection; diagnosis; detection syphilis ... See more keywords
Droplet-Based Microfluidics Digital PCR for the Detection of KRAS Mutations.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6734-6_12
Abstract: We demonstrate an accurate and sensitive quantification of mutated KRAS oncogene in genomic DNA, using droplet-based microfluidics and digital PCR. read more here.
Keywords: detection kras; based microfluidics; microfluidics digital; digital pcr ... See more keywords
Detection and Measurement of Chimeric RNAs by RT-PCR.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9904-0_6
Abstract: Reverse-transcription polymerase chain reaction (RT-PCR) is a powerful combination of assays useful in detection and measurement of expressed RNA transcripts. RT-PCR consists of RNA isolation and reverse transcription into complementary DNA (cDNA), which can then… read more here.
Keywords: detection; measurement chimeric; detection measurement; pcr ... See more keywords
Assessment of NS1 gene-specific real time quantitative TaqMan PCR for the detection of Human Bocavirus in respiratory samples.
Sign Up to like & getrecommendations! Published in 2017 at "Molecular and cellular probes"
DOI: 10.1016/j.mcp.2017.04.005
Abstract: Human Bocaviruses (HBoV) were associated with respiratory diseases. Here, we assessed a TaqMan®-based PCR for the detection of all four HBoV subtype infections with a sensitivity up to 15 copies/reaction. To evaluate this assay on… read more here.
Keywords: ns1 gene; taqman; assessment ns1; pcr detection ... See more keywords
PCR Detection of Oxacillinases in Bacteria.
Sign Up to like & getrecommendations! Published in 2020 at "Microbial drug resistance"
DOI: 10.1089/mdr.2019.0330
Abstract: Oxacillinases (OXA) have been mostly described in Enterobacteriaceae, Acinetobacter, and Pseudomonas species. Recent years have witnessed an increased prevalence of intrinsic and/or acquired β-lactamase-producing Acinetobacter in food-producing animals. This study was conducted to assess the… read more here.
Keywords: oxacillinases bacteria; presence; pcr detection; detection oxacillinases ... See more keywords
Polymerase Chain Reaction (PCR) Detection of 3C Ligation Products Present in 3C, ChIP-Loop, and Control Libraries: Library Titration and Interaction Frequency Analysis.
Sign Up to like & getrecommendations! Published in 2018 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot097873
Abstract: In all 3C-based assays, the aim is to measure the frequency with which two loci interact within cells. This is achieved by determining the relative abundance of ligation products formed during the 3C protocol that… read more here.
Keywords: interaction; ligation products; ligation; pcr detection ... See more keywords
PCR Detection of HHV8 DNA in the Saliva of Removable Denture Wearers Compared to Dentate Cases in Shiraz, South of Iran
Sign Up to like & getrecommendations! Published in 2020 at "BioMed Research International"
DOI: 10.1155/2020/9358947
Abstract: Result In the denture wearers, HHV8 DNA was detected in 11 cases. Two of the controls amplified HHV8 DNA. Fisher's exact test demonstrates a significant difference between virus infection and using removable dentures (p =… read more here.
Keywords: denture; denture wearers; hhv8 dna; pcr detection ... See more keywords
A diagnostic test of real-time PCR detection in the diagnosis of clinical bloodstream infection.
Sign Up to like & getrecommendations! Published in 2022 at "Annals of palliative medicine"
DOI: 10.21037/apm-22-1071
Abstract: BACKGROUND Blood culture remains the standard for diagnosing bloodstream infections, but it is difficult to identify bacteria directly and timeliness. The real-time polymerase chain reaction (PCR) has the potential to fill this diagnostic gap. This… read more here.
Keywords: blood; blood culture; bloodstream; pcr detection ... See more keywords
Evaluation of Two Assays Immunochromatography and RT-PCR for detection of Rotavirus in Iraqi Children
Sign Up to like & getrecommendations! Published in 2023 at "Research Journal of Pharmacy and Technology"
DOI: 10.52711/0974-360x.2023.00223
Abstract: The lack of dedicated RV treatment makes early detection and effective vaccines important to prevent increased mortality and morbidity, as they can only be treated with fluid and electrolyte replacement. The study's goal was to… read more here.
Keywords: detection rotavirus; rotavirus; detection; pcr detection ... See more keywords