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   Articles with "pcr hrm" as a keyword



Enhanced Commendable Sensitivity and Specificity for MSI in Colorectal Cancer by a New PCR‐HRM Based 8‐Loci MSI Assay

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recommendations! Published in 2024 at "Journal of Clinical Laboratory Analysis"

DOI: 10.1002/jcla.25085

Abstract: This study evaluated the performance of the PCR‐HRM assay by comparing it with immunohistochemistry (IHC) for mismatch repair (MMR) proteins and the PCR capillary electrophoresis (PCR‐CE) methods. read more here.

Keywords: commendable sensitivity; pcr; sensitivity specificity; enhanced commendable ... See more keywords

Simultaneous Detection of Three Genotypes of Gene Methylene Tetrahydrofolate Reductase and Methionine Synthase Reductase Based on Multiplex Asymmetric Real-Time PCR-HRM Biosensing.

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recommendations! Published in 2022 at "Analytical chemistry"

DOI: 10.1021/acs.analchem.2c02096

Abstract: Genotyping of folate metabolism genes is of great importance in disease diagnosis and prevention. However, most current detection methods used for folate metabolism gene genotyping are based on sequencing and chips, which suffer from a… read more here.

Keywords: folate metabolism; multiplex asymmetric; detection; reductase ... See more keywords

Efficient differentiation between Salmonella Pullorum and Salmonella Gallinarum by a fimH-based PCR-HRM

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recommendations! Published in 2025 at "Avian Pathology"

DOI: 10.1080/03079457.2025.2450840

Abstract: ABSTRACT Salmonella Pullorum (S. Pullorum) and Salmonella Gallinarum (S. Gallinarum) are the biovars of Salmonella enterica serovar Gallinarum that are responsible for pullorum disease and fowl typhoid, respectively, in poultry. Traditional serological methods fail to… read more here.

Keywords: differentiation; gallinarum; pullorum; salmonella pullorum ... See more keywords

Development and application of a PCR-HRM molecular diagnostic method of SNPs linked with TNF inhibitor efficacy

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recommendations! Published in 2018 at "Diagnosis"

DOI: 10.1515/dx-2018-0062

Abstract: Abstract Background Clinical evidence indicates that genetic variations may interfere with the mechanism of drug action. Recently, it has been reported that the single nucleotide polymorphisms (SNPs) of STAT4, PTPN2, PSORS1C1 and TRAF3IP2RA genes are… read more here.

Keywords: pcr hrm; snps; method; efficacy ... See more keywords

Comparison of Direct Sequencing, Real-Time PCR-High Resolution Melt (PCR-HRM) and PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) Analysis for Genotyping of Common Thiopurine Intolerant Variant Alleles NUDT15 c.415C>T and TPMT c.719A>G (TPMT*3C)

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recommendations! Published in 2017 at "Diagnostics"

DOI: 10.3390/diagnostics7020027

Abstract: Thiopurine intolerance and treatment-related toxicity, such as fatal myelosuppression, is related to non-function genetic variants encoding thiopurine S-methyltransferase (TPMT) and Nudix hydrolase 15 (NUDT15). Genetic testing of the common variants NUDT15:NM_018283.2:c.415C>T (Arg139Cys, dbSNP rs116855232 T… read more here.

Keywords: pcr hrm; real time; time pcr; hrm pcr ... See more keywords