Simultaneous Detection of Three Genotypes of Gene Methylene Tetrahydrofolate Reductase and Methionine Synthase Reductase Based on Multiplex Asymmetric Real-Time PCR-HRM Biosensing.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c02096
Abstract: Genotyping of folate metabolism genes is of great importance in disease diagnosis and prevention. However, most current detection methods used for folate metabolism gene genotyping are based on sequencing and chips, which suffer from a… read more here.
Keywords: folate metabolism; multiplex asymmetric; detection; reductase ... See more keywords
Development and application of a PCR-HRM molecular diagnostic method of SNPs linked with TNF inhibitor efficacy
Sign Up to like & getrecommendations! Published in 2018 at "Diagnosis"
DOI: 10.1515/dx-2018-0062
Abstract: Abstract Background Clinical evidence indicates that genetic variations may interfere with the mechanism of drug action. Recently, it has been reported that the single nucleotide polymorphisms (SNPs) of STAT4, PTPN2, PSORS1C1 and TRAF3IP2RA genes are… read more here.
Keywords: pcr hrm; snps; method; efficacy ... See more keywords
Comparison of Direct Sequencing, Real-Time PCR-High Resolution Melt (PCR-HRM) and PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) Analysis for Genotyping of Common Thiopurine Intolerant Variant Alleles NUDT15 c.415C>T and TPMT c.719A>G (TPMT*3C)
Sign Up to like & getrecommendations! Published in 2017 at "Diagnostics"
DOI: 10.3390/diagnostics7020027
Abstract: Thiopurine intolerance and treatment-related toxicity, such as fatal myelosuppression, is related to non-function genetic variants encoding thiopurine S-methyltransferase (TPMT) and Nudix hydrolase 15 (NUDT15). Genetic testing of the common variants NUDT15:NM_018283.2:c.415C>T (Arg139Cys, dbSNP rs116855232 T… read more here.
Keywords: pcr hrm; real time; time pcr; hrm pcr ... See more keywords