A Multiplex-PCR Method for Diagnosis of AY-Group Phytoplasmas.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8837-2_11
Abstract: Polymerase chain reaction (PCR) methods using phytoplasma-specific primers are widely used to detect phytoplasmas from infected plants and insects. Here, I describe a method of multiplex-PCR to amplify nine gene fragments in PCR reactions from… read more here.
Keywords: method; pcr; group phytoplasmas; multiplex pcr ... See more keywords
A PCR method to detect mating types of Cochliobolus heterostrophus
Sign Up to like & getrecommendations! Published in 2018 at "Canadian Journal of Plant Pathology"
DOI: 10.1080/07060661.2018.1481884
Abstract: Abstract A rapid and sensitive polymerase chain reaction (PCR) method was established for detection of mating types of Cochliobolus heterostrophus, the causal agent of southern corn leaf blight (SCLB). Two pairs of mating type-specific primers… read more here.
Keywords: dna; corn; cochliobolus heterostrophus; mating types ... See more keywords
A triplex real-time PCR method to detect African swine fever virus gene-deleted and wild type strains
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Veterinary Science"
DOI: 10.3389/fvets.2022.943099
Abstract: Currently there is still no effective vaccines and drugs available for African swine fever virus (ASFV), a life-threatening virus to domestic pigs and wild boars. Therefore, accurate diagnosis is important for the prevention and control… read more here.
Keywords: triplex real; time pcr; real time; method ... See more keywords
Identification of Torquetenovirus Species in Patients with Kawasaki Disease Using a Newly Developed Species-Specific PCR Method
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24108674
Abstract: A next-generation sequencing (NGS) study identified a very high viral load of Torquetenovirus (TTV) in KD patients. We aimed to evaluate the feasibility of a newly developed quantitative species-specific TTV-PCR (ssTTV-PCR) method to identify the… read more here.
Keywords: ssttv pcr; pcr; newly developed; species specific ... See more keywords
Detecting the Neuraminidase R294K Mutation in Avian Influenza A (H7N9) Virus Using Reverse Transcription Droplet Digital PCR Method
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DOI: 10.3390/v15040983
Abstract: The R294K mutation in neuraminidase (NA) causes resistance to oseltamivir in the avian influenza virus H7N9. Reverse transcription droplet digital polymerase chain reaction (RT-dd PCR) is a novel technique for detecting single-nucleotide polymorphisms. This study… read more here.
Keywords: h7n9; r294k mutation; pcr method;
Detection of genetically modified soybeans by PCR method and immunoassay kits
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Food and Drug Analysis"
DOI: 10.38212/2224-6614.2788
Abstract: The suitability of detecting genetically modified (GM) soybeans by polymerase chain reaction (PCR) and immunoassay kits is dete rmined. Primers specific for inserted genes in the Roundup Ready TM soybean (RRS, Monsanto company) were applied.… read more here.
Keywords: immunoassay kits; modified soybeans; method immunoassay; genetically modified ... See more keywords