A Multiplex-PCR Method for Diagnosis of AY-Group Phytoplasmas.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8837-2_11
Abstract: Polymerase chain reaction (PCR) methods using phytoplasma-specific primers are widely used to detect phytoplasmas from infected plants and insects. Here, I describe a method of multiplex-PCR to amplify nine gene fragments in PCR reactions from… read more here.
Keywords: method; pcr; group phytoplasmas; multiplex pcr ... See more keywords
A PCR method to detect mating types of Cochliobolus heterostrophus
Sign Up to like & getrecommendations! Published in 2018 at "Canadian Journal of Plant Pathology"
DOI: 10.1080/07060661.2018.1481884
Abstract: Abstract A rapid and sensitive polymerase chain reaction (PCR) method was established for detection of mating types of Cochliobolus heterostrophus, the causal agent of southern corn leaf blight (SCLB). Two pairs of mating type-specific primers… read more here.
Keywords: dna; corn; cochliobolus heterostrophus; mating types ... See more keywords
Multiplex fluorescent amplification-refractory mutation system PCR method for the detection of 10 genetic defects in Holstein cattle and its comparison with the KASP genotyping assay.
Sign Up to like & getrecommendations! Published in 2024 at "Animal genetics"
DOI: 10.1111/age.13432
Abstract: The common deleterious genetic defects in Holstein cattle include haplotypes 1-6 (HH1-HH6), haplotypes for cholesterol deficiency (HCD), bovine leukocyte adhesion deficiency (BLAD), complex vertebral malformation (CVM) and brachyspina syndrome (BS). Recessive inheritance patterns of these… read more here.
Keywords: detection; defects holstein; holstein cattle; genetic defects ... See more keywords
A TaqMan Duplex Quantitative PCR Method for Detecting Klebsiella pneumoniae has Been Developed Based on Pan-Genome Analysis
Sign Up to like & getrecommendations! Published in 2025 at "Sains Malaysiana"
DOI: 10.17576/jsm-2025-5403-12
Abstract: Klebsiella pneumoniae is a significant pathogen capable of causing infections in the respiratory, urinary, and bloodstream. In this study, bioinformatics-based pan-genome analysis identified specific and conserved LptD and MerR protein gene sequences in K. pneumoniae.… read more here.
Keywords: method; pneumoniae; quantitative pcr; pcr method ... See more keywords
[Development of a PCR Method to Detect a Mysid Species Causing False Positives in Tests for Crustacean Allergens].
Sign Up to like & getrecommendations! Published in 2024 at "Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan"
DOI: 10.3358/shokueishi.65.48
Abstract: Mysids are small crustaceans that are closely related to shrimp/prawns and crabs but not subject to food allergen labeling requirements for raw materials. In the past, a processed food that contained Japanese smelt (wakasagi) was… read more here.
Keywords: method; pcr method; method detect; japanese smelt ... See more keywords
Real-time quantitative PCR method for assessing wheat cultivars for resistance to Zymoseptoria tritici
Sign Up to like & getrecommendations! Published in 2025 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2025.1605156
Abstract: Septoria tritici blotch, caused by Zymoseptoria tritici (formerly Mycosphaerella graminicola), is an economically significant disease of wheat (Triticum aestivum) worldwide. However, there is little understanding of the fungus’ growth dynamics during the 14- to 18-day… read more here.
Keywords: pcr method; wheat cultivars; real time; time ... See more keywords
A triplex real-time PCR method to detect African swine fever virus gene-deleted and wild type strains
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Veterinary Science"
DOI: 10.3389/fvets.2022.943099
Abstract: Currently there is still no effective vaccines and drugs available for African swine fever virus (ASFV), a life-threatening virus to domestic pigs and wild boars. Therefore, accurate diagnosis is important for the prevention and control… read more here.
Keywords: triplex real; time pcr; real time; method ... See more keywords
Performance Evaluation of a Commercial Real-Time PCR Method for the Detection of Lupin Traces in Food
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DOI: 10.3390/foods13040609
Abstract: In accordance with U.S. FDA Foods Program Regulatory Science Steering Committee guidelines, with this study, we optimized and validated a commercial real-time PCR method for the detection of low amounts of lupin in four classes… read more here.
Keywords: time pcr; pcr method; real time; detection ... See more keywords
Identification of Torquetenovirus Species in Patients with Kawasaki Disease Using a Newly Developed Species-Specific PCR Method
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24108674
Abstract: A next-generation sequencing (NGS) study identified a very high viral load of Torquetenovirus (TTV) in KD patients. We aimed to evaluate the feasibility of a newly developed quantitative species-specific TTV-PCR (ssTTV-PCR) method to identify the… read more here.
Keywords: ssttv pcr; pcr; newly developed; species specific ... See more keywords
Detecting the Neuraminidase R294K Mutation in Avian Influenza A (H7N9) Virus Using Reverse Transcription Droplet Digital PCR Method
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DOI: 10.3390/v15040983
Abstract: The R294K mutation in neuraminidase (NA) causes resistance to oseltamivir in the avian influenza virus H7N9. Reverse transcription droplet digital polymerase chain reaction (RT-dd PCR) is a novel technique for detecting single-nucleotide polymorphisms. This study… read more here.
Keywords: h7n9; r294k mutation; pcr method;
Detection of genetically modified soybeans by PCR method and immunoassay kits
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Food and Drug Analysis"
DOI: 10.38212/2224-6614.2788
Abstract: The suitability of detecting genetically modified (GM) soybeans by polymerase chain reaction (PCR) and immunoassay kits is dete rmined. Primers specific for inserted genes in the Roundup Ready TM soybean (RRS, Monsanto company) were applied.… read more here.
Keywords: immunoassay kits; modified soybeans; method immunoassay; genetically modified ... See more keywords