Comparative analysis of DNA extraction and PCR product purification methods for cervicovaginal microbiome analysis using cpn60 microbial profiling
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DOI: 10.1371/journal.pone.0262355
Abstract: Background The microbiota of the lower female genital tract plays an important role in women’s health. Microbial profiling using the chaperonin60 (cpn60) universal target (UT) improves resolution of vaginal species associated with negative health outcomes… read more here.
Keywords: purification; dna; microbial profiling; extraction ... See more keywords
Agarose gel electrophoresis to assess PCR product yield: comparison with spectrophotometry, fluorometry and qPCR.
Sign Up to like & getrecommendations! Published in 2022 at "BioTechniques"
DOI: 10.2144/btn-2021-0094
Abstract: Agarose gel electrophoresis is a relatively easy to use method, commonly applied to evaluate PCR reaction success. Intercalating agents or dyes are used to visualize the amplified fragments. However, it is uncertain to what extent… read more here.
Keywords: gel electrophoresis; pcr product; agarose gel;