Identification of three Asian otter species (Aonyx cinereus, Lutra sumatrana, and Lutrogale perspicillata) using a novel noninvasive PCR‐RFLP analysis
Sign Up to like & getrecommendations! Published in 2022 at "Ecology and Evolution"
DOI: 10.1002/ece3.9585
Abstract: Abstract Four species of otters occur in tropical Asia, and all face multiple threats to their survival. Studies of distribution and population trends of these otter species in Asia, where they occur sympatrically, are complicated… read more here.
Keywords: tanad mod; rflp; pcr rflp; otr nd4 ... See more keywords
Detecting the polymorphism of TERF1 gene by an improved PCR‐RFLP method
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Clinical Laboratory Analysis"
DOI: 10.1002/jcla.22171
Abstract: Polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) is a common and mature method of detecting the single nucleotide polymorphism (SNP). But, for the polymorphism site rs3863242 of telomeric repeat binding factor 1(TERF1) gene, there is… read more here.
Keywords: pcr rflp; terf1 gene; method; polymorphism ... See more keywords
Chronic incurable mastitis in sheep: prevalence, identification of predisposing factors, and genotyping of fungal causative species using PCR-RFLP.
Sign Up to like & getrecommendations! Published in 2021 at "Tropical animal health and production"
DOI: 10.1007/s11250-021-02703-5
Abstract: Mycotic mastitis is a neglected problem type of incurable chronic mastitis in sheep flock of many countries which associated with wide economic burden. In the current study, a total of 600 ewes at Menofia governorate,… read more here.
Keywords: mastitis; prevalence; mycotic mastitis; pcr rflp ... See more keywords
Molecular Detection and Differentiation of Different Theileria Species in Naturally Infected Goats Using Nested PCR-RFLP: A First Report from Northern India.
Sign Up to like & getrecommendations! Published in 2022 at "Acta parasitologica"
DOI: 10.1007/s11686-022-00553-1
Abstract: PURPOSE Theileriosis is an economically important tick-borne pathogen with a serious impact on livestock health and productivity. Despite the fact that bovine theileriosis has been widely investigated, there exists a paucity of information on these… read more here.
Keywords: pcr rflp; different theileria; rflp; goats using ... See more keywords
Molecular identification of biwa trout (Oncorhynchus masou rhodurus) using PCR–RFLP method
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Food Science and Technology"
DOI: 10.1007/s13197-019-03914-3
Abstract: Polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) analysis was used to identify meat from biwa trout (Oncorhynchus masou rhodurus), amago trout (Oncorhynchus masou ishikawae), yamame trout (Oncorhynchus masou masou), and rainbow trout (Oncorhynchus mykiss). PCR… read more here.
Keywords: pcr rflp; trout oncorhynchus; oncorhynchus masou; biwa trout ... See more keywords
Use of a gyrB PCR-RFLP method to diagnose tuberculosis and identify the causative Mycobacterium sp. in cattle and humans.
Sign Up to like & getrecommendations! Published in 2022 at "Comparative immunology, microbiology and infectious diseases"
DOI: 10.1016/j.cimid.2022.101767
Abstract: GyrB PCR-restriction fragment length polymorphism (RFLP) could be applied to diagnose bovine and human tuberculosis and detect the causative agent. The lymph nodes and lungs from 50 cattle positive in tuberculin skin test were examined… read more here.
Keywords: tuberculosis; histopathology; gyrb pcr; pcr rflp ... See more keywords
PCR-RFLP for authentication of different species of processed snappers using mitochondrial D-loop region by single enzyme
Sign Up to like & getrecommendations! Published in 2018 at "Food Control"
DOI: 10.1016/j.foodcont.2018.02.028
Abstract: Abstract A PCR-RFLP protocol was developed for authenticating nine different snapper species by targeting the 515 bp fragment in highly polymorphic mitochondrial D-loop region with a newly designed primer Fish-DL-F/Fish-DL-R, followed by restriction digestion using… read more here.
Keywords: pcr rflp; loop region; single enzyme; mitochondrial loop ... See more keywords
Identification of Major SARS CoV-2 Variants in India using in silico PCR-RFLP analysis
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Infectious Diseases"
DOI: 10.1016/j.ijid.2021.12.083
Abstract: Purpose As of June 30, 2021, WHO reported 181,344,224 confirmed cases worldwide and 3,934,252 deaths due to SARS CoV-2. India accounts almost 10% of the total mortality. The mutated Variants of Concern (VoC) and Variants… read more here.
Keywords: pcr rflp; sars cov; major sars; cov variants ... See more keywords
In silico design and in vitro validation of a novel PCR-RFLP assay for determination of phylogenetic clusters of streptokinase gene alleles in streptococci groups.
Sign Up to like & getrecommendations! Published in 2019 at "Microbial pathogenesis"
DOI: 10.1016/j.micpath.2019.103862
Abstract: Streptokinase (SK), a heterogeneous plasminogen (Pg) activator protein secreted by groups A, C and G streptococci (GAS/GCS/GGS) is a virulence factor composed of three structural domains; SKα/SKβ/SKγ. Phylogenetic analysis of the major variable region of… read more here.
Keywords: analysis; novel pcr; rflp; rflp assay ... See more keywords
Identification of Spodoptera frugiperda (Lepidoptera: Noctuidae) and its two host strains in China by PCR-RFLP.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of economic entomology"
DOI: 10.1093/jee/toad073
Abstract: The fall armyworm (FAW) Spodoptera frugiperda was first found in China in 2018. In other countries, FAW has evolved corn and rice strain biotypes. It is not possible to identify these strains based on morphology.… read more here.
Keywords: faw; spodoptera; strain; pcr rflp ... See more keywords
A Simple PCR-RFLP Method for Genotyping of IFNL4 rs368234815 Polymorphism in Patients With Chronic Hepatitis C
Sign Up to like & getrecommendations! Published in 2017 at "Laboratory Medicine"
DOI: 10.1093/labmed/lmw060
Abstract: Background: The IFNL4 rs368234815 polymorphism plays a prominent role in spontaneous and treatment-induced clearance of hepatitis C virus (HCV) infection. This study aimed to develop a polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) method for… read more here.
Keywords: pcr rflp; rs368234815 polymorphism; method; polymorphism ... See more keywords