Exploring protocol bias in airway microbiome studies: one versus two PCR steps and 16S rRNA gene region V3 V4 versus V4
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DOI: 10.1186/s12864-020-07252-z
Abstract: Background Studies on the airway microbiome have been performed using a wide range of laboratory protocols for high-throughput sequencing of the bacterial 16S ribosomal RNA (16S rRNA) gene. We sought to determine the impact of… read more here.
Keywords: airway microbiome; pcr steps; number; 16s rrna ... See more keywords