DMSO and betaine significantly enhance the PCR amplification of ITS2 DNA barcodes from plants.
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DOI: 10.1139/gen-2019-0221
Abstract: ITS2 marker is highly efficient in species discrimination but its application in DNA barcoding is limited due to huge variations in the PCR success rate. We have hypothesized that higher GC content and the resultant… read more here.
Keywords: success rate; betaine; pcr success; pcr amplification ... See more keywords