Improving the specific activity and thermo-stability of alkaline pectate lyase from Bacillus subtilis 168 for bioscouring
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical Engineering Journal"
DOI: 10.1016/j.bej.2017.11.001
Abstract: Abstract Biocatalysts requires enzymes with high activity and stability under process conditions for efficient application. Several protein improvement strategies were used to improve pectate lyase PEL168 from Bacillus subtilis. Initially, a rationally designed mutant V132F… read more here.
Keywords: specific activity; k47d v132f; pectate lyase; stability ... See more keywords
Biochemical characterization and immobilization of a novel pectate lyase ErPL2 for efficient preparation of pectin oligosaccharides.
Sign Up to like & getrecommendations! Published in 2022 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2022.02.022
Abstract: Pectate lyase (ErPL2) from Echinicola rosea JL3085 showed maximal activity at 45 °C and pH 9.0 with 0.6 mM CaCl2. The Km and Vmax values of ErPL2 for polygalacturonic sodium were 2.098 mmol/L and 0.955 mmol/s, respectively. ErPL2 endolytically degraded… read more here.
Keywords: lyase erpl2; biochemical characterization; pectate lyase; characterization immobilization ... See more keywords
FvWRKY48 binds to the pectate lyase FvPLA promoter to control fruit softening in Fragaria vesca.
Sign Up to like & getrecommendations! Published in 2022 at "Plant physiology"
DOI: 10.1093/plphys/kiac091
Abstract: The regulatory mechanisms that link WRKY gene expression to fruit ripening are largely unknown. Using transgenic approaches, we showed that a WRKY gene from wild strawberry (Fragaria vesca), FvWRKY48, may be involved in fruit softening… read more here.
Keywords: fruit softening; lyase fvpla; fruit; pectate lyase ... See more keywords
A Rice PECTATE LYASE-LIKE Gene Is Required for Plant Growth and Leaf Senescence1[OPEN]
Sign Up to like & getrecommendations! Published in 2017 at "Plant Physiology"
DOI: 10.1104/pp.16.01625
Abstract: DEL1 affects rice growth and leaf senescence mediated by PECTATE LYASE-LIKE genes. To better understand the molecular mechanisms behind plant growth and leaf senescence in monocot plants, we identified a mutant exhibiting dwarfism and an… read more here.
Keywords: growth leaf; plant; senescence; pectate lyase ... See more keywords
Pectate Lyase from Fusarium sacchari Induces Plant Immune Responses and Contributes to Virulence
Sign Up to like & getrecommendations! Published in 2023 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.00165-23
Abstract: Pokkah Boeng disease (PBD) is one of the main diseases affecting sugarcane in China, seriously damaging sugarcane production and economic development. Therefore, it is important to clarify the pathogenic mechanisms of this disease and to… read more here.
Keywords: plant; sugarcane; virulence; pectate lyase ... See more keywords
Rational design and structure-based engineering of alkaline pectate lyase from Paenibacillus sp. 0602 to improve thermostability
Sign Up to like & getrecommendations! Published in 2021 at "BMC Biotechnology"
DOI: 10.1186/s12896-021-00693-8
Abstract: Background Ramie degumming is often carried out at high temperatures; therefore, thermostable alkaline pectate lyase (PL) is beneficial for ramie degumming for industrial applications. Thermostable PLs are usually obtained by exploring new enzymes or reconstructing… read more here.
Keywords: engineering; alkaline pectate; thermostability; rational design ... See more keywords
High-Level Expression and Biochemical Properties of A Thermo-Alkaline Pectate Lyase From Bacillus sp. RN1 in Pichia pastoris With Potential in Ramie Degumming
Sign Up to like & getrecommendations! Published in 2020 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2020.00850
Abstract: Pectate lyases play an essential role in textiles, animal feed, and oil extraction industries. Pichia pastoris can be an ideal platform for pectate lyases production, and BspPel (a thermo-alkaline pectate lyase from Bacillus sp. RN1)… read more here.
Keywords: pectate; thermo alkaline; alkaline pectate; pectate lyase ... See more keywords
A Meloidogyne graminicola Pectate Lyase Is Involved in Virulence and Activation of Host Defense Responses
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2021.651627
Abstract: Plant-parasitic nematodes secrete an array of cell-wall-degrading enzymes to overcome the physical barrier formed by the plant cell wall. Here, we describe a novel pectate lyase gene Mg-PEL1 from M. graminicola. Quantitative real-time PCR assay… read more here.
Keywords: pectate lyase; pel1; defense responses; plant ... See more keywords
Characterization of a Novel Creeping Tartary Buckwheat (Fagopyrum tataricum) Mutant lazy1
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2022.815131
Abstract: Gravity is known as an important environmental factor involved in the regulation of plant architecture. To identify genes related to the gravitropism of Tartary buckwheat, a creeping line was obtained and designated as lazy1 from… read more here.
Keywords: lazy1; tartary buckwheat; cell; pectate lyase ... See more keywords
A Novel PL9 Pectate Lyase from Paenibacillus polymyxa KF-1: Cloning, Expression, and Its Application in Pectin Degradation
Sign Up to like & getrecommendations! Published in 2019 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms20123060
Abstract: Pectate lyases play an important role in pectin degradation, and therefore are highly useful in the food and textile industries. Here, we report on the cloning of an alkaline pectate lyase gene (pppel9a) from Paenibacillus… read more here.
Keywords: pectate lyase; pectate; pectin; pectin degradation ... See more keywords
Structure of an Alkaline Pectate Lyase and Rational Engineering with Improved Thermo-Alkaline Stability for Efficient Ramie Degumming
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24010538
Abstract: Alkaline pectate lyases have biotechnological applications in plant fiber processing, such as ramie degumming. Previously, we characterized an alkaline pectate lyase from Bacillus clausii S10, named BacPelA, which showed potential for enzymatic ramie degumming because… read more here.
Keywords: ramie degumming; structure; pectate lyase; ramie ... See more keywords