Optimization of penicillin G acylase immobilized on glutaraldehyde‐modified titanium dioxide
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology and Applied Biochemistry"
DOI: 10.1002/bab.1817
Abstract: In this work, TiO2, which was modified by glutaraldehyde, was adopted as the carrier; the penicillin G acylase (PGA) was immobilized and the influence of immobilized conditions, such as pH of solution, the concentration of… read more here.
Keywords: penicillin acylase; immobilized pga; penicillin; optimization penicillin ... See more keywords
Incorporating Lanthanum into Mesoporous Silica Foam Enhances Enzyme Immobilization and the Activity of Penicillin G Acylase Due to Lewis Acid‐Base Interactions
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DOI: 10.1002/cbic.201900753
Abstract: Penicillin G acylase (PGA) has been immobilized on a lanthanum‐incorporated mesostructured cellular foam (La‐MCF) support by using the interaction between the strong Lewis acid sites on the surface of La‐MCF and the free amino groups… read more here.
Keywords: pga mcf; penicillin acylase; lewis acid; activity ... See more keywords
Computational design of penicillin acylase variants with improved kinetic selectivity for the enzymatic synthesis of cefazolin
Sign Up to like & getrecommendations! Published in 2021 at "Biochemical Engineering Journal"
DOI: 10.1016/j.bej.2021.108149
Abstract: Abstract Enzymatic synthesis plays a pivotal role in the sustainable production of semi-synthetic β-lactam antibiotics. However, these types of green syntheses are being developed slowly because of a lack of appropriate enzymes. In the present… read more here.
Keywords: enzymatic synthesis; synthesis; kinetic selectivity; penicillin acylase ... See more keywords
Computational redesign of penicillin acylase for cephradine synthesis with high kinetic selectivity
Sign Up to like & getrecommendations! Published in 2018 at "Green Chemistry"
DOI: 10.1039/c8gc03420f
Abstract: Computational redesign of native enzyme active sites for non-natural substrates offers a general approach for creating biocatalysts in green chemistry. Herein, a computational strategy was developed to discover selective mutants of penicillin acylase to catalyze… read more here.
Keywords: penicillin acylase; chemistry; cephradine; computational redesign ... See more keywords
Production and Application of Penicillin G Acylase: A Brief Update.
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DOI: 10.2174/1389201020666191111151642
Abstract: BACKGROUND Penicillins and cephalosporins are the β-lactam antibiotics most used worldwide for therapeutic treatment of different bacterial infections. Currently, both drugs are produced using penicillin G acylase (PGA) as biocatalyst. OBJECTIVE The objective of this… read more here.
Keywords: production; lactam antibiotics; penicillin acylase; application ... See more keywords
Strategies to Improve the Biosynthesis of β-Lactam Antibiotics by Penicillin G Acylase: Progress and Prospects
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.936487
Abstract: β-Lactam antibiotics are widely used anti-infection drugs that are traditionally synthesized via a chemical process. In recent years, with the growing demand for green alternatives, scientists have turned to enzymatic synthesis. Penicillin G acylase (PGA)… read more here.
Keywords: improve biosynthesis; penicillin acylase; lactam antibiotics; biosynthesis lactam ... See more keywords
Penicillin Acylase from Streptomyces lavendulae and Aculeacin A Acylase from Actinoplanes utahensis: Two Versatile Enzymes as Useful Tools for Quorum Quenching Processes
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DOI: 10.3390/catal10070730
Abstract: Many Gram-negative bacteria produce N-acyl-homoserine lactones (AHLs), quorum sensing (QS) molecules that can be enzymatically inactivated by quorum quenching (QQ) processes; this approach is considered an emerging antimicrobial alternative. In this study, kinetic parameters of… read more here.
Keywords: aculeacin acylase; hsl; quenching processes; penicillin acylase ... See more keywords
Immobilization of Penicillin G Acylase on Vinyl Sulfone-Agarose: An Unexpected Effect of the Ionic Strength on the Performance of the Immobilization Process
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DOI: 10.3390/molecules27217587
Abstract: Penicillin G acylase (PGA) from Escherichia coli was immobilized on vinyl sulfone (VS) agarose. The immobilization of the enzyme failed at all pH values using 50 mM of buffer, while the progressive increase of ionic… read more here.
Keywords: immobilization; sulfone agarose; penicillin acylase; vinyl sulfone ... See more keywords