Improved assay system for acidic peptide: N-glycanase (aPNGase) activity in plant extracts.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2021.114367
Abstract: PLANT ACIDIC PEPTIDE N-glycanase (aPNGase) release N-glycans from glycopeptides during the degradation process of glycoproteins in developing or growing plants. We have previously developed a new method to detect the aPNGase activity in crude extracts,… read more here.
Keywords: apngase activity; glycanase apngase; apngase; acidic peptide ... See more keywords
Protein Sequence Editing of SKN-1A/Nrf1 by Peptide:N-Glycanase Controls Proteasome Gene Expression
Sign Up to like & getrecommendations! Published in 2019 at "Cell"
DOI: 10.1016/j.cell.2019.03.035
Abstract: The proteasome mediates selective protein degradation and is dynamically regulated in response to proteotoxic challenges. SKN-1A/Nrf1, an endoplasmic reticulum (ER)-associated transcription factor that undergoes N-linked glycosylation, serves as a sensor of proteasome dysfunction and triggers… read more here.
Keywords: sequence editing; protein sequence; peptide glycanase; skn nrf1 ... See more keywords
Luminescence detection of peptide:N-glycanase activity using engineered split inteins.
Sign Up to like & getrecommendations! Published in 2022 at "Chemical communications"
DOI: 10.1039/d2cc04865e
Abstract: A split intein-based method has been developed to detect peptide:N-glycanase (PNGase) activity in live cells. PNGase cleaves the linkage between N,N'-diacetylchitobiose and the Asn side-chain of N-intein peptides and the products react rapidly with C-intein… read more here.
Keywords: luminescence detection; split; detection peptide; activity ... See more keywords