MS Ana: Improving Sensitivity in Peptide Identification with Spectral Library Search
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Proteome Research"
DOI: 10.1021/acs.jproteome.2c00658
Abstract: Spectral library search can enable more sensitive peptide identification in tandem mass spectrometry experiments. However, its drawbacks are the limited availability of high-quality libraries and the added difficulty of creating decoy spectra for result validation.… read more here.
Keywords: sensitivity peptide; library search; peptide identification; spectral library ... See more keywords
Single-Molecule Peptide Identification Using Fluorescence Blinking Fingerprints
Sign Up to like & getrecommendations! Published in 2023 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c12561
Abstract: The ability to identify peptides with single-molecule sensitivity would lead to next-generation proteomics methods for basic research and clinical applications. Existing single-molecule peptide sequencing methods can read some amino acid sequences, but they are limited… read more here.
Keywords: single molecule; molecule; peptide identification; identification using ... See more keywords
Evaluation and optimization of reduction and alkylation methods to maximize peptide identification with MS-based proteomics.
Sign Up to like & getrecommendations! Published in 2017 at "Molecular bioSystems"
DOI: 10.1039/c7mb00393e
Abstract: Mass spectrometry (MS) has become an increasingly important technique to analyze proteins. In popular bottom-up MS-based proteomics, reduction and alkylation are routine steps to facilitate peptide identification. However, incomplete reactions and side reactions may occur,… read more here.
Keywords: reduction; based proteomics; reduction alkylation; alkylation ... See more keywords
SW-Tandem: a highly efficient tool for large-scale peptide identification with parallel spectrum dot product on Sunway TaihuLight
Sign Up to like & getrecommendations! Published in 2019 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btz147
Abstract: SUMMARY Tandem mass spectrometry (MS/MS) based database searching is a widely acknowledged and adopted method that identifies peptide sequence in shotgun proteomics. However, database searching is extremely computationally expensive, which can take days even weeks… read more here.
Keywords: large scale; tool; sunway taihulight; peptide identification ... See more keywords
Improvement of peptide identification with considering the abundance of mRNA and peptide
Sign Up to like & getrecommendations! Published in 2017 at "BMC Bioinformatics"
DOI: 10.1186/s12859-017-1491-5
Abstract: BackgroundTandem mass spectrometry (MS/MS) followed by database search is a main approach to identify peptides/proteins in proteomic studies. A lot of effort has been devoted to improve the identification accuracy and sensitivity for peptides/proteins, such… read more here.
Keywords: identification; abundance; improvement peptide; peptide identification ... See more keywords