Optimal analytical strategies for sensitive and quantitative phosphoproteomics using TMT‐based multiplexing
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DOI: 10.1002/pmic.202100245
Abstract: In large‐scale quantitative mass spectrometry (MS)‐based phosphoproteomics, isobaric labeling with tandem mass tags (TMTs) coupled with offline high‐pH reversed‐phase peptide chromatographic fractionation maximizes depth of coverage. To investigate to what extent limited sample amounts affect… read more here.
Keywords: tmt based; sample; peptide input; per sample ... See more keywords