High‐Throughput Screening of Signal Peptide Library with Novel Fluorescent Probe
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DOI: 10.1002/cbic.202100523
Abstract: Nitrile hydratase (NHase) is an excellent biocatalyst for the synthesis of amide compounds and is composed of two heterologous subunits. However, the secretory expression of NHase has been difficult to achieve because of its complex… read more here.
Keywords: peptide library; fluorescent probe; throughput screening; high throughput ... See more keywords
cDNA Display of Disulfide-Containing Peptide Library and In Vitro Evolution.
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DOI: 10.1007/978-1-4939-9853-1_4
Abstract: Directed in vitro evolution (IVE) is now a widely applied technology to obtain molecules that have designed new features of one's demands. We describe here experimental procedures for a cDNA display IVE to select peptide… read more here.
Keywords: evolution; vitro evolution; peptide library; disulfide containing ... See more keywords
Discovery of Surfactant-Like Peptides from a Phage-Displayed Peptide Library
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DOI: 10.3390/v12121442
Abstract: Peptides with specific affinities for various materials have been identified in the past three decades and utilized in materials science and engineering. A peptide’s capability to specifically interact with materials is not naturally derived but… read more here.
Keywords: peptides phage; surfactant like; displayed peptide; peptide library ... See more keywords
Analysis of Compositional Bias in a Commercial Phage Display Peptide Library by Next-Generation Sequencing
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DOI: 10.3390/v14112402
Abstract: The principal presumption of phage display biopanning is that the naïve library contains an unbiased repertoire of peptides, and thus, the enriched variants derive from the affinity selection of an entirely random peptide pool. In… read more here.
Keywords: peptide library; next generation; phage display; display peptide ... See more keywords