Challenges in the use of sortase and other peptide ligases for site-specific protein modification
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DOI: 10.1039/d0cs01148g
Abstract: Site-specific protein modification is a widely-used biochemical tool. However, there are many challenges associated with the development of protein modification techniques, in particular, achieving site-specificity, reaction efficiency and versatility. The engineering of peptide ligases and… read more here.
Keywords: site specific; site; protein modification; peptide ligases ... See more keywords
Structural characterisation of methanogen pseudomurein cell wall peptide ligases homologous to bacterial MurE/F murein peptide ligases.
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DOI: 10.1099/mic.0.001235
Abstract: Archaea have diverse cell wall types, yet none are identical to bacterial peptidoglycan (murein). Methanogens Methanobacteria and Methanopyrus possess cell walls of pseudomurein, a structural analogue of murein. Pseudomurein differs from murein in containing the… read more here.
Keywords: cell; bacterial mure; murein; peptide ligases ... See more keywords
Reversed Proteolysis—Proteases as Peptide Ligases
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DOI: 10.3390/catal11010033
Abstract: Historically, ligase activity by proteases was theoretically derived due to their catalyst nature, and it was experimentally observed as early as around 1900. Initially, the digestive proteases, such as pepsin, chymotrypsin, and trypsin were employed… read more here.
Keywords: proteolysis proteases; reversed proteolysis; proteases peptide; peptide ligases ... See more keywords