Peptide mapping of proteins by capillary electromigration methods.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of separation science"
DOI: 10.1002/jssc.202200664
Abstract: This review article provides a wide overview of important developments and applications of capillary electromigration methods in the area of peptide mapping of proteins in the period 1997-mid 2022, including review articles on this topic.… read more here.
Keywords: capillary electromigration; electromigration methods; proteins capillary; electromigration ... See more keywords
Understanding the pathway and kinetics of aspartic acid isomerization in peptide mapping methods for monoclonal antibodies
Sign Up to like & getrecommendations! Published in 2021 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-021-03176-z
Abstract: Isomerization of aspartic acid (Asp) in therapeutic proteins could lead to safety and efficacy concerns. Thus, accurate quantitation of various Asp isomerization along with kinetic understanding of the variant formations is needed to ensure optimal… read more here.
Keywords: aspartic acid; understanding pathway; isomerization; pathway kinetics ... See more keywords
Characterization and quantification of succinimide using peptide mapping under low-pH conditions and hydrophobic interaction chromatography.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2018.11.021
Abstract: Characterization of asparagine deamidation and aspartic acid isomerization is an important aspect of biotherapeutic protein analysis due to the potential negative effect of these modifications on drug efficacy and stability. Succinimide has long been known… read more here.
Keywords: hydrophobic interaction; mapping low; interaction chromatography; peptide mapping ... See more keywords
Site-Specific Conjugation Quantitation of a Cysteine-Conjugated Antibody-Drug Conjugate Using Stable Isotope Labeling Peptide Mapping LC-MS/MS Analysis.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c04025
Abstract: Drug-load (DL) characterization of antibody-drug conjugates (ADCs) is an important analytical task due to its designation as a critical quality attribute (CQA) affecting potency and stability. Intact and subunit liquid chromatography-mass spectrometry (LC-MS) analyses can… read more here.
Keywords: site specific; conjugation; drug; antibody drug ... See more keywords
Pertuzumab Charge Variant Analysis and Complementarity-Determining Region Stability Assessment to Deamidation
Sign Up to like & getrecommendations! Published in 2023 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.2c03275
Abstract: Pertuzumab is a monoclonal antibody used for the treatment of HER2-positive breast cancer in combination with trastuzumab. Charge variants of trastuzumab have been extensively described in the literature; however, little is known about the charge… read more here.
Keywords: heavy chain; stress; deamidation; charge ... See more keywords
High-Resolution Capillary Zone Electrophoresis with Mass Spectrometry Peptide Mapping of Therapeutic Proteins: Peptide Recovery and Post-translational Modification Analysis in Monoclonal Antibodies and Antibody-Drug Conjugates.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.7b03643
Abstract: Reversed phase liquid chromatography with mass spectrometry (RPLC-MS) peptide mapping is routinely used for interrogating molecular and structural attributes such as amino acid composition, sequence variants, and post-translational modifications (PTMs) in antibody-derived therapeutics. RPLC has… read more here.
Keywords: peptide mapping; analysis; drug; mass spectrometry ... See more keywords
Antibody characterization using novel ERLIC-MS/MS-based peptide mapping
Sign Up to like & getrecommendations! Published in 2018 at "mAbs"
DOI: 10.1080/19420862.2018.1505179
Abstract: ABSTRACT Electrostatic repulsion hydrophilic interaction chromatography (ERLIC) coupled with mass spectrometry (MS) is a technique that is increasingly being used as a trapping/enrichment tool for glycopeptides/phosphorylated peptides or sample fractionation in proteomics research. Here, we… read more here.
Keywords: novel erlic; method; erlic based; based peptide ... See more keywords
Native peptide mapping – A simple method to routinely monitor higher order structure changes and relation to functional activity
Sign Up to like & getrecommendations! Published in 2019 at "mAbs"
DOI: 10.1080/19420862.2019.1634460
Abstract: ABSTRACT In the biopharmaceutical environment, controlling the Critical Quality Attributes (CQA) of a product is essential to prevent changes that affect its safety or efficacy. Physico-chemical techniques and bioassays are used to screen and monitor… read more here.
Keywords: higher order; structure; peptide mapping; activity ... See more keywords
Peptide Mapping, In Silico and In Vivo Analysis of Allergenic Sorghum Profilin Peptides
Sign Up to like & getrecommendations! Published in 2019 at "Medicina"
DOI: 10.3390/medicina55050178
Abstract: Background and objectives: Nearly 20–30% of the world’s population suffers from allergic rhinitis, among them 15% are progressing to asthma conditions. Sorghum bicolor profilin (Sorb PF), one of the panallergens, was identified, but the allergen… read more here.
Keywords: silico vivo; profilin; mapping silico; sorghum profilin ... See more keywords