Anthranilic Acid Accumulation in Saccharomyces cerevisiae Induced by Expression of a Nonribosomal Peptide Synthetase Gene from Paecilomyces cinnamomeus BCC 9616
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200573
Abstract: Heterologous expression of nrps33, a nonribosomal peptide synthetase gene, from Paecilomyces cinnamomeus BCC 9616 in Saccharomyces cerevisiae unexpectedly resulted in the accumulation of anthranilic acid, an intermediate in tryptophan biosynthesis. Based on transcriptomic and real‐time… read more here.
Keywords: peptide synthetase; synthetase gene; anthranilic acid; accumulation ... See more keywords
Comparison of actinomycin peptide synthetase formation in Streptomyces chrysomallus and Streptomyces antibioticus
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Basic Microbiology"
DOI: 10.1002/jobm.201800244
Abstract: Actinomycin peptide synthetase genes constitute two oppositely oriented transcriptional units, acmADR, and acmBC, separated by a non‐coding intergenic region. Gene constructs of the intergenic region together with its adjoining gene acmA or acmB from the… read more here.
Keywords: peptide synthetase; formation; streptomyces chrysomallus; gene ... See more keywords
Aspcandine: A Pyrrolobenzazepine Alkaloid Synthesized by a Fungal Nonribosomal Peptide Synthetase-Polyketide Synthase Hybrid.
Sign Up to like & getrecommendations! Published in 2022 at "Organic letters"
DOI: 10.1021/acs.orglett.2c01918
Abstract: Characterization of an orphan biosynthetic gene cluster found in the fungus Aspergillus candidus CBS 102.13 resulted in the discovery of a pyrrolobenzazepine alkaloid, aspcandine (1). The unique molecular scaffold of 1 is synthesized by the… read more here.
Keywords: peptide synthetase; pyrrolobenzazepine alkaloid; synthetase polyketide; polyketide synthase ... See more keywords
Biosynthesis of Tetronates by a Nonribosomal Peptide Synthetase-Polyketide Synthase System.
Sign Up to like & getrecommendations! Published in 2023 at "Organic letters"
DOI: 10.1021/acs.orglett.3c00103
Abstract: A cryptic tetronate biosynthetic pathway was identified in Kitasatospora niigatensis DSM 44781 via heterologous expression. Distinct from the currently known biosynthetic pathways, this system utilizes a partially functional nonribosomal peptide synthetase and a broadly selective… read more here.
Keywords: nonribosomal peptide; peptide synthetase; system; polyketide synthase ... See more keywords
Recruitment and Regulation of the Non-ribosomal Peptide Synthetase Modifying Cytochrome P450 Involved in Nikkomycin Biosynthesis.
Sign Up to like & getrecommendations! Published in 2017 at "ACS chemical biology"
DOI: 10.1021/acschembio.7b00081
Abstract: The β-hydroxylation of l-histidine is the first step in the biosynthesis of the imidazolone base of the antifungal drug nikkomycin. The cytochrome P450 (NikQ) hydroxylates the amino acid while it is appended via a phosphopantetheine… read more here.
Keywords: biosynthesis; ribosomal peptide; amino acid; peptide synthetase ... See more keywords
FRET monitoring of a nonribosomal peptide synthetase.
Sign Up to like & getrecommendations! Published in 2017 at "Nature chemical biology"
DOI: 10.1038/nchembio.2435
Abstract: Nonribosomal peptide synthetases (NRPSs) are multidomain enzyme templates for the synthesis of bioactive peptides. Large-scale conformational changes during peptide assembly are obvious from crystal structures, yet their dynamics and coupling to catalysis are poorly understood.… read more here.
Keywords: peptide synthetase; fret monitoring; phenylalanine; monitoring nonribosomal ... See more keywords
Predicting the chemical space of fungal polyketides by phylogeny-based bioinformatics analysis of polyketide synthase-nonribosomal peptide synthetase and its modification enzymes
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DOI: 10.1038/s41598-020-70177-w
Abstract: Fungal polyketide synthase (PKS)–nonribosomal peptide synthetase (NRPS) hybrids are key enzymes for synthesizing structurally diverse hybrid natural products (NPs) with characteristic biological activities. Predicting their chemical space is of particular importance in the field of… read more here.
Keywords: space; peptide synthetase; analysis; chemical space ... See more keywords
An unusual Burkholderia gladioli double chain-initiating nonribosomal peptide synthetase assembles ‘fungal’ icosalide antibiotics† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c8sc04897e
Sign Up to like & getrecommendations! Published in 2019 at "Chemical Science"
DOI: 10.1039/c8sc04897e
Abstract: Fungus-associated Burkholderia gladioli bacteria use a unique ‘dual-priming’ nonribosomal peptide synthetase to assemble icosalide A1. read more here.
Keywords: nonribosomal peptide; peptide synthetase; burkholderia gladioli;
A Highly Conserved Basidiomycete Peptide Synthetase Produces a Trimeric Hydroxamate Siderophore
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DOI: 10.1128/aem.01478-17
Abstract: ABSTRACT The model white-rot basidiomycete, Ceriporiopsis (Gelatoporia) subvermispora B, encodes putative natural product biosynthesis genes. Among them is the gene for the seven-domain nonribosomal peptide synthetase CsNPS2. It is a member of the as-yet-uncharacterized fungal… read more here.
Keywords: peptide synthetase; csnps2; conserved basidiomycete; siderophore ... See more keywords