Quantitative Measurement of Cholesterol in Cell Populations Using Flow Cytometry and Fluorescent Perfringolysin O.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6875-6_8
Abstract: Methods to quantify intracellular cholesterol are valuable for the study of its trafficking and storage in normal cells and in lysosomal storage disorders. Traditionally, cholesterol has been tracked using the small molecule, filipin. Filipin can… read more here.
Keywords: perfringolysin; quantitative measurement; cell populations; measurement cholesterol ... See more keywords
Mechanistic Insights into the Cholesterol-dependent Binding of Perfringolysin O-based Probes and Cell Membranes
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DOI: 10.1038/s41598-017-14002-x
Abstract: Cholesterol distribution in the cell is maintained by both vesicular and non-vesicular sterol transport. Non-vesicular transport is mediated by the interaction of membrane-embedded cholesterol and water-soluble proteins. Small changes to the lipid composition of the… read more here.
Keywords: cholesterol content; perfringolysin; cholesterol; cholesterol dependent ... See more keywords
Piceatannol Alleviates Clostridium perfringens Virulence by Inhibiting Perfringolysin O
Sign Up to like & getrecommendations! Published in 2022 at "Molecules"
DOI: 10.3390/molecules27165145
Abstract: Clostridium perfringens (C. perfringens) is an important foodborne pathogen that can cause diseases such as gas gangrene and necrotizing enteritis in a variety of economic animals, seriously affecting public health and the economic benefits and… read more here.
Keywords: perfringolysin; piceatannol alleviates; virulence; clostridium perfringens ... See more keywords