Excess copper catalyzes protein disulfide bond formation in the bacterial periplasm but not in the cytoplasm
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DOI: 10.1111/mmi.15032
Abstract: Copper avidly binds thiols and is redox active, and it follows that one element of copper toxicity may be the generation of undesirable disulfide bonds in proteins. In the present study, copper oxidized the model… read more here.
Keywords: periplasm; copper; cytoplasm; excess copper ... See more keywords
Expression of Soluble Active Interferon αA in Escherichia coli Periplasm by Fusion with Thermostable Lichenase Using the Domain Insertion Approach
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DOI: 10.1134/s0006297918030069
Abstract: A recombinant DNA in which the interferon αA (IFN–αA) gene sequence is integrated into a loop region of the gene coding thermostable lichenase was constructed. This approach of insertion fusion with thermostable lichenase is advantageous… read more here.
Keywords: periplasm; insertion; lichenase; thermostable lichenase ... See more keywords
Adaptation of the periplasm to maintain spatial constraints essential for cell envelope processes and cell viability
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DOI: 10.7554/elife.73516
Abstract: The cell envelope of Gram-negative bacteria consists of two membranes surrounding a periplasm and peptidoglycan layer. Molecular machines spanning the cell envelope depend on spatial constraints and load-bearing forces across the cell envelope and surface.… read more here.
Keywords: periplasm; cell; viability; spatial constraints ... See more keywords