A Thermostable Heme Protein Fold Adapted for Stereoselective C−H Bond Hydroxylation Using Peroxygenase Activity
Sign Up to like & getrecommendations! Published in 2024 at "ChemBioChem"
DOI: 10.1002/cbic.202400737
Abstract: Thermostable protein folds of natural and synthetic origin are highly sought‐after templates for biocatalyst generation due to their enhanced stability to elevated temperatures which overcomes one of the major limitations of applying enzymes for synthesis.… read more here.
Keywords: protein fold; protein; stereoselective bond; heme ... See more keywords
Broadening the Catalytic Scope of the Peroxygenase Activity of a Bacterial Tyrosine Hydroxylase
Sign Up to like & getrecommendations! Published in 2024 at "ChemCatChem"
DOI: 10.1002/cctc.202401819
Abstract: Fungal unspecific peroxygenases (UPOs, EC 1.11.2.1) are known for their unique biocatalytic properties and have been extensively studied since their discovery more than two decades ago. UPOs have found a wide range of applications in… read more here.
Keywords: tyrosine hydroxylase; scope; broadening catalytic; peroxygenase ... See more keywords
Peroxidase versus Peroxygenase Activity: Substrate Substituent Effects as Modulators of Enzyme Function in the Multifunctional Catalytic Globin Dehaloperoxidase.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemistry"
DOI: 10.1021/acs.biochem.8b00540
Abstract: The dehaloperoxidase-hemoglobin (DHP) from the terebellid polychaete Amphitrite ornata is a multifunctional hemoprotein that catalyzes the oxidation of a wide variety of substrates, including halo/nitrophenols, haloindoles, and pyrroles, via peroxidase and/or peroxygenase mechanisms. To probe… read more here.
Keywords: peroxidase; multifunctional catalytic; substituent effects; substrate substituent ... See more keywords
Structure-Guided Engineering of CYP109B4: From Monooxygenase to Peroxygenase for Selective Steroid Hydroxylation.
Sign Up to like & getrecommendations! Published in 2025 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.5c11530
Abstract: Cytochrome P450 CYP109B4 is a characterized 16β-steroid hydroxylase with significant potential in steroid drug biosynthesis, but its application is limited by reliance on costly coenzymes and complex electron transfer processes. To address this, we constructed… read more here.
Keywords: structure guided; guided engineering; cyp109b4; peroxygenase ... See more keywords
Peroxide-Driven Hydroxylation of Small Alkanes Catalyzed by an Artificial P450BM3 Peroxygenase System
Sign Up to like & getrecommendations! Published in 2019 at "ACS Catalysis"
DOI: 10.1021/acscatal.9b02507
Abstract: We report the selective hydroxylation of small alkanes with H2O2 catalyzed by an artificial P450 peroxygenase system generated from engineered cytochrome P450BM3 variants in assistance with dual-functional small molecule (DFSM), in which DFSM acts as… read more here.
Keywords: system; peroxygenase; small alkanes; hydroxylation small ... See more keywords
Fenton-like Chemistry by a Copper(I) Complex and H2O2 Relevant to Enzyme Peroxygenase C-H Hydroxylation.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.3c02273
Abstract: Lytic polysaccharide monooxygenases have received significant attention as catalytic convertors of biomass to biofuel. Recent studies suggest that its peroxygenase activity (i.e., using H2O2 as an oxidant) is more important than its monooxygenase functionality. Here,… read more here.
Keywords: copper complex; chemistry; hydroxylation; h2o2 ... See more keywords
A Well-Defined Osmium-Cupin Complex: Hyperstable Artificial Osmium Peroxygenase.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.7b00675
Abstract: Thermally stable TM1459 cupin superfamily protein from Thermotoga maritima was repurposed as an osmium (Os) peroxygenase by metal-substitution strategy employing the metal-binding promiscuity. This novel artificial metalloenzyme bears a datively bound Os ion supported by… read more here.
Keywords: well defined; donor set; cupin; peroxygenase ... See more keywords
Reaction engineering blocks ether cleavage for synthesizing chiral cyclic hemiacetals catalyzed by unspecific peroxygenase
Sign Up to like & getrecommendations! Published in 2024 at "Nature Communications"
DOI: 10.1038/s41467-024-45545-z
Abstract: Hemiacetal compounds are valuable building blocks in synthetic chemistry, but their enzymatic synthesis is limited and often hindered by the instability of hemiacetals in aqueous environments. Here, we show that this challenge can be addressed… read more here.
Keywords: reaction; cyclic hemiacetals; reaction engineering; peroxygenase ... See more keywords
Specific Caleosin/Peroxygenase and Lipoxygenase Activities Are Tissue-Differentially Expressed in Date Palm (Phoenix dactylifera L.) Seedlings and Are Further Induced Following Exposure to the Toxin 2,3,7,8-tetrachlorodibenzo-p-dioxin
Sign Up to like & getrecommendations! Published in 2017 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2016.02025
Abstract: Two caleosin/peroxygenase isoforms from date palm, Phoenix dactylifera L., PdCLO2 and PdCLO4, were characterized with respect to their tissue expression, subcellular localization, and oxylipin pathway substrate specificities in developing seedlings. Both PdCLO2 and PdCLO4 had… read more here.
Keywords: toxin; peroxygenase; date palm; exposure ... See more keywords
Engineering Collariella virescens Peroxygenase for Epoxides Production from Vegetable Oil
Sign Up to like & getrecommendations! Published in 2022 at "Antioxidants"
DOI: 10.3390/antiox11050915
Abstract: Vegetable oils are valuable renewable resources for the production of bio-based chemicals and intermediates, including reactive epoxides of industrial interest. Enzymes are an environmentally friendly alternative to chemical catalysis in oxygenation reactions, epoxidation included, with… read more here.
Keywords: oil; collariella virescens; epoxidation; production ... See more keywords
Inhibition of the Peroxygenase Lytic Polysaccharide Monooxygenase by Carboxylic Acids and Amino Acids
Sign Up to like & getrecommendations! Published in 2022 at "Antioxidants"
DOI: 10.3390/antiox11061096
Abstract: Lytic polysaccharide monooxygenases (LPMOs) are widely distributed in fungi, and catalyze the oxidative degradation of polysaccharides such as cellulose. Despite their name, LPMOs possess a dominant peroxygenase activity that is reflected in high turnover numbers… read more here.
Keywords: lytic polysaccharide; inhibition; activity; peroxygenase ... See more keywords