System-level perturbations of cell metabolism using CRISPR/Cas9.
Sign Up to like & getrecommendations! Published in 2017 at "Current opinion in biotechnology"
DOI: 10.1016/j.copbio.2017.03.014
Abstract: CRISPR/Cas9 (clustered regularly interspaced palindromic repeats and the associated protein Cas9) techniques have made genome engineering and transcriptional reprogramming studies more advanced and cost-effective. For metabolic engineering purposes, the CRISPR-based tools have been applied to… read more here.
Keywords: level perturbations; crispr cas9; system level; cell metabolism ... See more keywords
Perturbations in cell signaling elicit early cardiac defects in mucopolysaccharidosis type II
Sign Up to like & getrecommendations! Published in 2017 at "Human Molecular Genetics"
DOI: 10.1093/hmg/ddx069
Abstract: &NA; Morphogens release and activity can be negatively affected by an impaired glycosaminoglycans (GAGs) turnover and proteoglycans assembly in the extracellular matrix, leading to altered tissue morphogenesis. In this work, we show that loss of… read more here.
Keywords: mucopolysaccharidosis type; signaling elicit; elicit early; cell signaling ... See more keywords
Biomolecular Perturbations in In-Cell Dynamic Nuclear Polarization Experiments
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2021.743829
Abstract: In-cell DNP is a growing application of NMR to the study of biomolecular structure and function within intact cells. An important unresolved question for in-cell DNP spectroscopy is the integrity of cellular samples under the… read more here.
Keywords: cell dnp; biomolecular perturbations; preservation; dnp ... See more keywords