Ancestral Sequence Reconstruction and Comprehensive Computational Simulations Unmask an Efficient PET Hydrolase with the Wobbled Catalytic Triad.
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DOI: 10.1002/cssc.202402614
Abstract: Beyond directed evolution, ancestral sequence reconstruction (ASR) has emerged as a powerful strategy for engineering proteins with superior functional properties. Herein, we harnessed ASR to uncover robust PET hydrolase variants, expanding the repertoire of PET-degrading… read more here.
Keywords: sequence; ancestral sequence; pet hydrolase; sequence reconstruction ... See more keywords
Cellular Upcycling of Polyethylene Terephthalate (PET) With an Engineered Human Saliva Metagenomic PET Hydrolase.
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DOI: 10.1002/cssc.202502560
Abstract: Recent advances in biocatalytic recycling of polyethylene terephthalate (PET) using PET hydrolase enzymes have sparked interest in integrating PET degradation capabilities into living systems. Although cell‐based strategies are limited by the mesophilic temperature constraints of… read more here.
Keywords: terephthalate pet; pet hydrolase; human saliva; polyethylene terephthalate ... See more keywords
In Silico Analysis and Biochemical Characterization of Streptomyces PET Hydrolase with Bis(2-Hydroxyethyl) Terephthalate Biodegradation Activity
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DOI: 10.4014/jmb.2404.04030
Abstract: Polyethylene terephthalate (PET), one of the most widely used plastics in the world, causes serious environmental problems. Recently, scientists have been focused on the enzymatic degradation of PET, an environmentally friendly method that offers an… read more here.
Keywords: hydrolase; bis hydroxyethyl; pet hydrolase; bhet ... See more keywords