Protein Crystallography and Site‐Direct Mutagenesis Analysis of the Poly(ethylene terephthalate) Hydrolase PETase from Ideonella sakaiensis
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DOI: 10.1002/cbic.201800097
Abstract: Unlike traditional recycling strategies, biodegradation is a sustainable solution for disposing of poly(ethylene terephthalate) (PET) waste. PETase, a newly identified enzyme from Ideonella sakaiensis, has high efficiency and specificity towards PET and is, thus, a… read more here.
Keywords: ideonella sakaiensis; poly ethylene; petase; ethylene terephthalate ... See more keywords
Enhancing Secretion of Polyethylene Terephthalate Hydrolase PETase in Bacillus subtilis WB600 Mediated by the SPamy Signal Peptide.
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DOI: 10.1111/lam.13312
Abstract: The polyethylene terephthalate hydrolase (PETase) has been proved to have a high activity to degrade polyethylene terephthalate(PET), but few studies has been carried on its secretion in Bacillus subtilis. In this study, the coding gene… read more here.
Keywords: terephthalate hydrolase; polyethylene terephthalate; secretion; hydrolase petase ... See more keywords
Hydrophobic cell surface display system of PETase as a sustainable biocatalyst for PET degradation
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DOI: 10.3389/fmicb.2022.1005480
Abstract: Remarkably, a hydrolase from Ideonella sakaiensis 201-F6, termed PETase, exhibits great potential in polyethylene terephthalate (PET) waste management due to it can efficiently degrade PET under moderate conditions. However, its low yield and poor accessibility… read more here.
Keywords: system; cell; pet; surface ... See more keywords
In Silico Identification of Potential Sites for a Plastic-Degrading Enzyme by a Reverse Screening through the Protein Sequence Space and Molecular Dynamics Simulations
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DOI: 10.3390/molecules27103353
Abstract: The accumulation of polyethylene terephthalate (PET) seriously harms the environment because of its high resistance to degradation. The recent discovery of the bacteria-secreted biodegradation enzyme, PETase, sheds light on PET recycling; however, the degradation efficiency… read more here.
Keywords: dynamics simulations; sequence space; molecular dynamics; petase ... See more keywords