A novel multiplex qPCR assay for detection of Plasmodium falciparum with histidine-rich protein 2 and 3 (pfhrp2 and pfhrp3) deletions in polyclonal infections
Sign Up to like & getrecommendations! Published in 2020 at "EBioMedicine"
DOI: 10.1016/j.ebiom.2020.102757
Abstract: Background Many health facilities in malaria endemic countries are dependent on Rapid diagnostic tests (RDTs) for diagnosis and some National Health Service (NHS) hospitals without expert microscopists rely on them for diagnosis out of hours.… read more here.
Keywords: protein; pfhrp2 pfhrp3; pfhrp3 deletions; qpcr assay ... See more keywords
Plasmodium falciparum histidine-rich protein (PfHRP2 and 3) diversity in Western and Coastal Kenya
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-018-38175-1
Abstract: Plasmodium falciparum histidine-rich proteins 2 (PfHRP2) based RDTs are advocated in falciparum malaria-endemic regions, particularly when quality microscopy is not available. However, diversity and any deletion in the pfhrp2 and pfhrp3 genes can affect the… read more here.
Keywords: pfhrp3; amino acid; pfhrp2 pfhrp3; plasmodium falciparum ... See more keywords
Systematic review of the status of pfhrp2 and pfhrp3 gene deletion, approaches and methods used for its estimation and reporting in Plasmodium falciparum populations in Africa: review of published studies 2010–2019
Sign Up to like & getrecommendations! Published in 2019 at "Malaria Journal"
DOI: 10.1186/s12936-019-2987-4
Abstract: BackgroundMalaria rapid diagnostic tests based on histidine-rich protein-2 have played a vital role in improving malaria case management and surveillance particularly in Africa, where Plasmodium falciparum is predominant. However, their usefulness has been threatened by… read more here.
Keywords: pfhrp2 pfhrp3; deletion; gene deletion; review ... See more keywords
Genetic polymorphism of histidine rich protein 2 in Plasmodium falciparum isolates from different infection sources in Yunnan Province, China
Sign Up to like & getrecommendations! Published in 2019 at "Malaria Journal"
DOI: 10.1186/s12936-019-3084-4
Abstract: BackgroundFailed diagnoses of some falciparum malaria cases by RDTs are constantly reported in recent years. Plasmodium falciparum histidine-rich protein 2 (pfhpr2) gene deficiency has been found to be the major reason of RDTs failure in… read more here.
Keywords: peptide; yunnan province; province; type ... See more keywords
Plasmodium falciparum histidine-rich protein 2 diversity in Ghana
Sign Up to like & getrecommendations! Published in 2020 at "Malaria Journal"
DOI: 10.1186/s12936-020-03328-z
Abstract: In the absence of microscopy, Plasmodium falciparum histidine-rich proteins 2 (PfHRP2)-based rapid diagnostic tests (RDTs) are recommended for the diagnosis of falciparum malaria, particularly in endemic regions. However, genetic variability of the pfhrp2 gene threatens… read more here.
Keywords: diversity; microscopy; falciparum; falciparum histidine ... See more keywords
Molecular surveillance reveals the presence of pfhrp2 and pfhrp3 gene deletions in Plasmodium falciparum parasite populations in Uganda, 2017–2019
Sign Up to like & getrecommendations! Published in 2020 at "Malaria Journal"
DOI: 10.1186/s12936-020-03362-x
Abstract: Histidine-rich protein-2 (HRP2)-based rapid diagnostic tests (RDTs) are the only RDTs recommended for malaria diagnosis in Uganda. However, the emergence of Plasmodium falciparum histidine rich protein 2 and 3 (pfhrp2 and pfhrp3) gene deletions threatens… read more here.
Keywords: gene deletions; pfhrp2 pfhrp3; presence; gene ... See more keywords
Limited threat of Plasmodium falciparum pfhrp2 and pfhrp3 gene deletion to the utility of HRP2-based malaria RDTs in Northern Uganda
Sign Up to like & getrecommendations! Published in 2024 at "Malaria Journal"
DOI: 10.1186/s12936-023-04830-w
Abstract: Background Rapid diagnostic tests (RDTs) that detect Plasmodium falciparum histidine-rich protein-2 (PfHRP2) are exclusively deployed in Uganda, but deletion of the pfhrp2/3 target gene threatens their usefulness as malaria diagnosis and surveillance tools. Methods A… read more here.
Keywords: pfhrp2; plasmodium; pfhrp3 gene; gene ... See more keywords
Widespread pfhrp2/3 deletions and HRP2-based false-negative results in southern Ethiopia
Sign Up to like & getrecommendations! Published in 2024 at "Malaria Journal"
DOI: 10.1186/s12936-024-04904-3
Abstract: Background Rapid diagnostic tests (RDTs) play a significant role in expanding case management in peripheral healthcare systems. Histidine-rich protein-2 (HRP2) antigen detection RDTs are predominantly used to diagnose Plasmodium falciparum infection. However, the evolution and… read more here.
Keywords: pfhrp2 deletions; pfhrp2; falciparum; false negative ... See more keywords
Novel Plasmodium falciparum histidine-rich protein 2/3 repeat type in Ethiopian malaria infection: does this affect performance of HRP2-based malaria RDT?
Sign Up to like & getrecommendations! Published in 2024 at "Malaria Journal"
DOI: 10.1186/s12936-024-05093-9
Abstract: Rapid diagnostic tests (RDTs) provide quick, easy, and convenient early diagnosis of malaria ensuring better case management particularly in resource-constrained settings. Nevertheless, the efficiency of HRP2-based RDT can be compromised by Plasmodium falciparum histidine-rich protein… read more here.
Keywords: repeat; pfhrp2; type; plasmodium falciparum ... See more keywords
First report of pfhrp2 and pfhrp3 gene deletions compromising HRP2-based malaria rapid diagnostic tests in Malawi
Sign Up to like & getrecommendations! Published in 2025 at "Infectious Diseases of Poverty"
DOI: 10.1186/s40249-025-01368-8
Abstract: Histidine Rich Protein 2-based rapid diagnostic tests (HRP2-based RDTs) are widely used for malaria diagnosis in Malawi, but their accuracy may be compromised by Plasmodium falciparum parasites lacking the P. falciparum histidine rich protein 2… read more here.
Keywords: pfhrp2; gene deletions; hrp2 based; pfhrp3 gene ... See more keywords
Application of egg yolk IgY on carboxylated polypyrrole films for impedimetric detection of PfHRP2 antigen.
Sign Up to like & getrecommendations! Published in 2022 at "Bioelectrochemistry"
DOI: 10.2139/ssrn.4191685
Abstract: This paper described an impedimetric immunosensor for detecting Plasmodium falciparum histidine-rich protein 2 (PfHRP2). Antibodies from egg yolk (Ab-PfHRP2, IgY type) were linked covalently to the screen-printed gold electrodes (SPGE) surface modified with a thin… read more here.
Keywords: application egg; spectroscopy; pfhrp2; egg yolk ... See more keywords