A novel multiplex qPCR assay for detection of Plasmodium falciparum with histidine-rich protein 2 and 3 (pfhrp2 and pfhrp3) deletions in polyclonal infections
Sign Up to like & getrecommendations! Published in 2020 at "EBioMedicine"
DOI: 10.1016/j.ebiom.2020.102757
Abstract: Background Many health facilities in malaria endemic countries are dependent on Rapid diagnostic tests (RDTs) for diagnosis and some National Health Service (NHS) hospitals without expert microscopists rely on them for diagnosis out of hours.… read more here.
Keywords: protein; pfhrp2 pfhrp3; pfhrp3 deletions; qpcr assay ... See more keywords
Plasmodium falciparum histidine-rich protein (PfHRP2 and 3) diversity in Western and Coastal Kenya
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DOI: 10.1038/s41598-018-38175-1
Abstract: Plasmodium falciparum histidine-rich proteins 2 (PfHRP2) based RDTs are advocated in falciparum malaria-endemic regions, particularly when quality microscopy is not available. However, diversity and any deletion in the pfhrp2 and pfhrp3 genes can affect the… read more here.
Keywords: pfhrp3; amino acid; pfhrp2 pfhrp3; plasmodium falciparum ... See more keywords
Systematic review of the status of pfhrp2 and pfhrp3 gene deletion, approaches and methods used for its estimation and reporting in Plasmodium falciparum populations in Africa: review of published studies 2010–2019
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DOI: 10.1186/s12936-019-2987-4
Abstract: BackgroundMalaria rapid diagnostic tests based on histidine-rich protein-2 have played a vital role in improving malaria case management and surveillance particularly in Africa, where Plasmodium falciparum is predominant. However, their usefulness has been threatened by… read more here.
Keywords: pfhrp2 pfhrp3; deletion; gene deletion; review ... See more keywords
Genetic polymorphism of histidine rich protein 2 in Plasmodium falciparum isolates from different infection sources in Yunnan Province, China
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DOI: 10.1186/s12936-019-3084-4
Abstract: BackgroundFailed diagnoses of some falciparum malaria cases by RDTs are constantly reported in recent years. Plasmodium falciparum histidine-rich protein 2 (pfhpr2) gene deficiency has been found to be the major reason of RDTs failure in… read more here.
Keywords: peptide; yunnan province; province; type ... See more keywords
Plasmodium falciparum histidine-rich protein 2 diversity in Ghana
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DOI: 10.1186/s12936-020-03328-z
Abstract: In the absence of microscopy, Plasmodium falciparum histidine-rich proteins 2 (PfHRP2)-based rapid diagnostic tests (RDTs) are recommended for the diagnosis of falciparum malaria, particularly in endemic regions. However, genetic variability of the pfhrp2 gene threatens… read more here.
Keywords: diversity; microscopy; falciparum; falciparum histidine ... See more keywords
Molecular surveillance reveals the presence of pfhrp2 and pfhrp3 gene deletions in Plasmodium falciparum parasite populations in Uganda, 2017–2019
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DOI: 10.1186/s12936-020-03362-x
Abstract: Histidine-rich protein-2 (HRP2)-based rapid diagnostic tests (RDTs) are the only RDTs recommended for malaria diagnosis in Uganda. However, the emergence of Plasmodium falciparum histidine rich protein 2 and 3 (pfhrp2 and pfhrp3) gene deletions threatens… read more here.
Keywords: gene deletions; pfhrp2 pfhrp3; presence; gene ... See more keywords
Application of egg yolk IgY on carboxylated polypyrrole films for impedimetric detection of PfHRP2 antigen.
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DOI: 10.2139/ssrn.4191685
Abstract: This paper described an impedimetric immunosensor for detecting Plasmodium falciparum histidine-rich protein 2 (PfHRP2). Antibodies from egg yolk (Ab-PfHRP2, IgY type) were linked covalently to the screen-printed gold electrodes (SPGE) surface modified with a thin… read more here.
Keywords: application egg; spectroscopy; pfhrp2; egg yolk ... See more keywords
Plasmodium falciparum pfhrp2 and pfhrp3 Gene Deletions from Persons with Symptomatic Malaria Infection in Ethiopia, Kenya, Madagascar, and Rwanda
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DOI: 10.3201/eid2803.211499
Abstract: Histidine-rich protein 2 (HRP2)–based rapid diagnostic tests detect Plasmodium falciparum malaria and are used throughout sub-Saharan Africa. However, deletions in the pfhrp2 and related pfhrp3 (pfhrp2/3) genes threaten use of these tests. Therapeutic efficacy studies… read more here.
Keywords: pfhrp2; madagascar; plasmodium; persons symptomatic ... See more keywords
Plasmodium falciparum pfhrp2 and pfhrp3 Gene Deletions and Relatedness to Other Global Isolates, Djibouti, 2019–2020
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DOI: 10.3201/eid2810.220695
Abstract: Deletions of pfhrp2 and paralogue pfhrp3 (pfhrp2/3) genes threaten Plasmodium falciparum diagnosis by rapid diagnostic test. We examined 1,002 samples from suspected malaria patients in Djibouti City, Djibouti, to investigate pfhrp2/3 deletions. We performed assays… read more here.
Keywords: pfhrp2; plasmodium falciparum; gene deletions; pfhrp2 pfhrp3 ... See more keywords
Prevalence of Pfhrp2/3 gene deletions among false negative rapid antigen test results in central India
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DOI: 10.4103/0972-9062.328815
Abstract: Background &objectives: The diagnosis of Plasmodium falciparum malaria is widely dependent on the P. falciparum histidine rich protein 2 (PfHRP2) antigens based rapid diagnostic tests. There are few possible factors like Pfhrp2 polymorphism, Pfhrp2 deletion… read more here.
Keywords: pfhrp2; falciparum; false negative; microscopy ... See more keywords